אסיף מאגר המחקר החקלאי

Nondestructive assessment of chlorophyll content in watercress leaves by a tristimulus reflectance colorimeter

Year:

1992

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Volume :

2

Co-Authors:

Meir, S., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Philosoph-Hadas, S., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Gloter, P., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Aharoni, N., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel

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Abstract:

In the present study we have applied the nondestructive system of the Commission International d'Eclairage (CIE) for color assessment in fruits to measure chlorophyll (Chl) in senescing leaves. The extractable Chl values of various watercress leaves at different senescence stages were correlated with their quantitative color specifications, based on CIE-L*a*b* values, by the use of reflectance spectroscopy. Experimental evidence was based on correlation of 46 determinations of extracted Chl values (mg Chl/g fw) in watercress leaves with their corresponding calculated CIE-Lab values and functions - hue angle (H°) and total color difference (ΔE). Results show that better correleations exist between Chl content in watercress leaves and values of b*, L* and the ΔE function, than for a* value and the H° function. The best correlation (R2 = 0.868) was obtained between the single function number of ΔE and the extractable Chl values. Based on this correlation, a formula was derived for routine and nondestructive evaluation of Chl content in senescing watercress leaves. © 1992.

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