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A transgenic tomato F1 hybrid harboring a defective viral replicase shows immunity to cucumber mosaic virus in field trials
Year:
1999
Source of publication :
Acta Horticulturae
Authors :
Gal-On, Amit
;
.
Pilowsky, Meir
;
.
Wolf, Dalia
;
.
Zelcer, Aaron
;
.
Volume :
487
Co-Authors:
Gal-On, A., Dept. of Viroloev, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Wolf, D., Dept. of Plant Genetics, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Pilowsky, M., Dept. of Plant Genetics, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Zelcer, A.
Facilitators :
From page:
329
To page:
333
(
Total pages:
5
)
Abstract:
Cucumber mosaic virus (CMV) epidemics occur worldwide in tomato fields, resulting in severe yield losses. Presently, no resistant commercial cultivars are available, since reponed sources of genetic resistance are unsatisfactory or genetically complex. We have recently described the generation of tomato lines which show immunity to CMV, following transformation with a truncated CMV replicase gene and subsequent screening of the Rl offsprings by mechanical inoculation (Gal-On et al, in press). A Fl hybrid was generated by crossing one of our most resistant lines with the appropriate complementary line. Field trials have indicated that deliberate inoculation with high titers of CMV resulted in ca. 50% reduction of yield in the non-transformedhybrid, while the transformed hybrid - hemizygous for the replicase gene - was unaffected and yielded normally.
Note:
Related Files :
Agrobacterium
CMV
Cucumber mosaic virus
ELISA
Lycopersicon esculentum
transformation
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28520
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:39
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Scientific Publication
A transgenic tomato F1 hybrid harboring a defective viral replicase shows immunity to cucumber mosaic virus in field trials
487
Gal-On, A., Dept. of Viroloev, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Wolf, D., Dept. of Plant Genetics, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Pilowsky, M., Dept. of Plant Genetics, Agricultural Research Organization, P.O.Box 6, Bet-Dagan, 50250, Israel
Zelcer, A.
A transgenic tomato F1 hybrid harboring a defective viral replicase shows immunity to cucumber mosaic virus in field trials
Cucumber mosaic virus (CMV) epidemics occur worldwide in tomato fields, resulting in severe yield losses. Presently, no resistant commercial cultivars are available, since reponed sources of genetic resistance are unsatisfactory or genetically complex. We have recently described the generation of tomato lines which show immunity to CMV, following transformation with a truncated CMV replicase gene and subsequent screening of the Rl offsprings by mechanical inoculation (Gal-On et al, in press). A Fl hybrid was generated by crossing one of our most resistant lines with the appropriate complementary line. Field trials have indicated that deliberate inoculation with high titers of CMV resulted in ca. 50% reduction of yield in the non-transformedhybrid, while the transformed hybrid - hemizygous for the replicase gene - was unaffected and yielded normally.
Scientific Publication
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