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Differential protein expression in Colletotrichum acutatum: Changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry
Year:
2008
Source of publication :
Molecular Plant Pathology
Authors :
Belausov, Eduard
;
.
Chen, Songbi
;
.
Freeman, Stanley
;
.
Gollop, Natan
;
.
Horowitz, Sigal Brown
;
.
Zveibil, Aida
;
.
Volume :
9
Co-Authors:

Brown, S.H., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Chen, S., Department of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel

Facilitators :
From page:
171
To page:
190
(
Total pages:
20
)
Abstract:
The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized during appressorium formation under nitrogen-limiting conditions compared with a complete nutrient supply. Proteins that were up- or down-regulated were involved in energy metabolism, nitrogen and amino acid metabolism, protein synthesis and degradation, response to stress and reactive oxygen scavenging. Members belonging to the reactive oxygen species (ROS) scavenger machinery, superoxide dismutase and glutathione peroxidase, were up-regulated at the appressorium formation stage, as well as under nitrogen-limiting conditions relative to growth with a complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressorium formation stage. Fungal ROS were detected within germinating conidia during host pre-penetration, penetration and colonization stages, accompanied by plant ROS, which were abundant in the apoplastic space. Application of exogenous antioxidants quenched ROS production and reduced the frequency of appressorium formation. Up-regulation in metabolic activity was detected during appressorium formation and nutrient deficiency compared with growth under complete nutrient supply. Enhanced levels of proteins related to the glyoxylate cycle and lipid metabolism (malate dehydrogenase, formate dehydrogenase and acetyl-CoA acetyltransferase) were observed at the appressorium formation stage, in contrast to down-regulation of isocitrate dehydrogenase. The present study demonstrates that appressoria formation processes, occurring under nutritional deprivation, are accompanied by metabolic shifts, and that ROS production is an early fungal response that may modulate initial stages of pathogen development. © 2007 Blackwell Publishing Ltd.
Note:
Related Files :
Colletotrichum
Fragaria
molecular genetics
proteomics
reactive oxygen species
Show More
Related Content
More details
DOI :
10.1111/j.1364-3703.2007.00454.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28543
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
You may also be interested in
Scientific Publication
Differential protein expression in Colletotrichum acutatum: Changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry
9

Brown, S.H., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Yarden, O., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Chen, S., Department of Food Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Zveibil, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel

Differential protein expression in Colletotrichum acutatum: Changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry
The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized during appressorium formation under nitrogen-limiting conditions compared with a complete nutrient supply. Proteins that were up- or down-regulated were involved in energy metabolism, nitrogen and amino acid metabolism, protein synthesis and degradation, response to stress and reactive oxygen scavenging. Members belonging to the reactive oxygen species (ROS) scavenger machinery, superoxide dismutase and glutathione peroxidase, were up-regulated at the appressorium formation stage, as well as under nitrogen-limiting conditions relative to growth with a complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressorium formation stage. Fungal ROS were detected within germinating conidia during host pre-penetration, penetration and colonization stages, accompanied by plant ROS, which were abundant in the apoplastic space. Application of exogenous antioxidants quenched ROS production and reduced the frequency of appressorium formation. Up-regulation in metabolic activity was detected during appressorium formation and nutrient deficiency compared with growth under complete nutrient supply. Enhanced levels of proteins related to the glyoxylate cycle and lipid metabolism (malate dehydrogenase, formate dehydrogenase and acetyl-CoA acetyltransferase) were observed at the appressorium formation stage, in contrast to down-regulation of isocitrate dehydrogenase. The present study demonstrates that appressoria formation processes, occurring under nutritional deprivation, are accompanied by metabolic shifts, and that ROS production is an early fungal response that may modulate initial stages of pathogen development. © 2007 Blackwell Publishing Ltd.
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