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Reciprocal transfer of male sterile and normal plasmons in Petunia
Year:
1984
Source of publication :
Theoretical and Applied Genetics
Authors :
Izhar, Shamay
;
.
Swartzberg, Dvora
;
.
Tabib, Yona
;
.
Volume :
68
Co-Authors:
Izhar, S., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Tabib, Y., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Swartzberg, D., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Facilitators :
From page:
455
To page:
457
(
Total pages:
3
)
Abstract:
The goal in this experiment was to achieve direct plasmon transfer via cell fusion. Two lines were used - a normal fertile line of P. hybrida, and a cytoplasmic male sterile (cms) line with the nuclear background of P. parodii. Two plants phenotypically similar to the original male sterile line were developed from protoplasts, but instead of being cms they were male fertile. On the other hand, two plants typical of the original normal line developed from protoplasts, but they were cms instead of fertile. Chromosome counts were done and in all cases the expected diploid number (=14) was found. Genetic analysis showed that sorting out of cms and fertile segregants was evident in the first and second backcross of the cms cybrids. The fertile type cybrids were stable fertile for several generations of selfing and proper backcrossing. These results are discussed in the light of an earlier fusion experiment in which these two parental lines were involved. © 1984 Springer-Verlag.
Note:
Related Files :
Petunia
Plasmon transfer
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Related Content
More details
DOI :
10.1007/BF00254819
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28563
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
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Scientific Publication
Reciprocal transfer of male sterile and normal plasmons in Petunia
68
Izhar, S., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Tabib, Y., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Swartzberg, D., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50-250, Israel
Reciprocal transfer of male sterile and normal plasmons in Petunia
The goal in this experiment was to achieve direct plasmon transfer via cell fusion. Two lines were used - a normal fertile line of P. hybrida, and a cytoplasmic male sterile (cms) line with the nuclear background of P. parodii. Two plants phenotypically similar to the original male sterile line were developed from protoplasts, but instead of being cms they were male fertile. On the other hand, two plants typical of the original normal line developed from protoplasts, but they were cms instead of fertile. Chromosome counts were done and in all cases the expected diploid number (=14) was found. Genetic analysis showed that sorting out of cms and fertile segregants was evident in the first and second backcross of the cms cybrids. The fertile type cybrids were stable fertile for several generations of selfing and proper backcrossing. These results are discussed in the light of an earlier fusion experiment in which these two parental lines were involved. © 1984 Springer-Verlag.
Scientific Publication
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