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Plant, Cell & Environment
BARG, R., Department of Plant Genetics, The Volcani Center, Bet Dagan, Israel
UMIEL, N., Division of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
NITZAN, Y., Department of Life Sciences, Bar-Han University, Ramat-Gan, Israel
Abstract. Non‐differentiated tissue cultures (calli) and differentiated tissues (shoots) of tobacco were found to differ in their sensitivity to chloramphenicol (CAP). This phenomenon is especially manifested in darkness and in an illumination regime lacking u.v. and blue light. When the latter are included, CAP's photodegradation products are shown to appear. It seems that one of the main photodegradation pathways is through the production of p‐nitro‐benzaldehyde (p‐NBA) which is further degraded. The possibility that either acetylation or physiological nitration of CAP is the cause for the differential tolerance was eliminated. The chromatographic and radiographic results indicate that in vivo degradation of CAP occurs both in calli and in shoot cultures. One of the in vivo degradation products is CAP‐base. Copyright © 1983, Wiley Blackwell. All rights reserved
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Fate of chloramphenicol in tobacco tissue culture under various light regimes
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BARG, R., Department of Plant Genetics, The Volcani Center, Bet Dagan, Israel
UMIEL, N., Division of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
NITZAN, Y., Department of Life Sciences, Bar-Han University, Ramat-Gan, Israel
Fate of chloramphenicol in tobacco tissue culture under various light regimes
Abstract. Non‐differentiated tissue cultures (calli) and differentiated tissues (shoots) of tobacco were found to differ in their sensitivity to chloramphenicol (CAP). This phenomenon is especially manifested in darkness and in an illumination regime lacking u.v. and blue light. When the latter are included, CAP's photodegradation products are shown to appear. It seems that one of the main photodegradation pathways is through the production of p‐nitro‐benzaldehyde (p‐NBA) which is further degraded. The possibility that either acetylation or physiological nitration of CAP is the cause for the differential tolerance was eliminated. The chromatographic and radiographic results indicate that in vivo degradation of CAP occurs both in calli and in shoot cultures. One of the in vivo degradation products is CAP‐base. Copyright © 1983, Wiley Blackwell. All rights reserved
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