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Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves
Year:
1993
Source of publication :
Plant Growth Regulation
Authors :
Aharoni, Nehemia
;
.
Philosoph-Hadas, Sonia
;
.
Volume :
12
Co-Authors:
Philosoph-Hadas, S., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Centre, Bet Dagan, 50250, Israel
Hadas, E., Départment of Biotechnology, Tel-Aviv University, Tel Aviv, 69011, Israel
Aharoni, N., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Centre, Bet Dagan, 50250, Israel
Facilitators :
From page:
71
To page:
78
(
Total pages:
8
)
Abstract:
A new monoclonal antibody (mAb) was generated against abscisic acid (ABA), and an indirect enzyme-linked immunosorbent assay (ELISA) using this mAb was developed for convenient quantitative analysis of ABA levels in rice leaf extracts. The mAb, raised against (+-)-ABA conjugated to bovine serum albumin (BSA) through its carboxyl group (C1), reacted preferentially with the (+)-ABA enantiomer, and equally well with both free and methyl-ester (+-)-ABA. Cross-reactivity with several ABA-related compounds was negligible. Linearity was obtained between 3 and 1000 pmo1 of (+)-ABA. The ABA-mAb was further used to quantitate pmol quantities of (+)-ABA in attached and detached rice leaves. Results obtained with such ELISA quantitation showed an increase in the free ABA content of detached rice leaves at progressive stages of senescence, which was regarded as a senescence-related response. This quantitation compared favorably with other presently used techniques for ABA determination, with regard to their detection limits, cost and assay time. The results suggest that the combination of a specific mAb with a sensitive ELISA technique is quite promising for quantitation of ABA. © 1993 Kluwer Academic Publishers.
Note:
Related Files :
attached and detached rice leaves
ELISA
free and conjugated abscisic acid
Leaf senescence
Monoclonal antibodies
Oryza saliva
Show More
Related Content
More details
DOI :
10.1007/BF00144585
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28583
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
Scientific Publication
Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves
12
Philosoph-Hadas, S., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Centre, Bet Dagan, 50250, Israel
Hadas, E., Départment of Biotechnology, Tel-Aviv University, Tel Aviv, 69011, Israel
Aharoni, N., Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Centre, Bet Dagan, 50250, Israel
Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves
A new monoclonal antibody (mAb) was generated against abscisic acid (ABA), and an indirect enzyme-linked immunosorbent assay (ELISA) using this mAb was developed for convenient quantitative analysis of ABA levels in rice leaf extracts. The mAb, raised against (+-)-ABA conjugated to bovine serum albumin (BSA) through its carboxyl group (C1), reacted preferentially with the (+)-ABA enantiomer, and equally well with both free and methyl-ester (+-)-ABA. Cross-reactivity with several ABA-related compounds was negligible. Linearity was obtained between 3 and 1000 pmo1 of (+)-ABA. The ABA-mAb was further used to quantitate pmol quantities of (+)-ABA in attached and detached rice leaves. Results obtained with such ELISA quantitation showed an increase in the free ABA content of detached rice leaves at progressive stages of senescence, which was regarded as a senescence-related response. This quantitation compared favorably with other presently used techniques for ABA determination, with regard to their detection limits, cost and assay time. The results suggest that the combination of a specific mAb with a sensitive ELISA technique is quite promising for quantitation of ABA. © 1993 Kluwer Academic Publishers.
Scientific Publication
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