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Ecdysone oxidase and 3‐oxoecdysteroid reductases in Manduca sexta midgut: Kinetic parameters
Year:
1989
Authors :
Svoboda, James
;
.
Volume :
12
Co-Authors:
Weirich, G.F., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Thompson, M.J., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Svoboda, J.A., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Facilitators :
From page:
201
To page:
218
(
Total pages:
18
)
Abstract:
Ecdysone and 20‐hydroxyecdysone are converted to their 3‐epimers by enzymes in the midgut cytosol of Manduca sexta larvae. A partially purified cytosol preparation has been used to analyze the nature of and the interaction between these enzymes. The cytosol was shown to contain ecdysone oxidase, one or more 3‐oxoecdysteroid 3α‐reductase(s), and one or more 3‐oxoecdysteroid 3β‐reductase(s). The reductases reacted at different velocities with NADH and NADPH. With NADH, 3α‐reduction was the major reaction; with NADPH, 3β‐reduction was the major reaction. The apparent kinetic parameters for the enzymes support the assumed two‐step mechanism for the 3‐epimerization with a 3‐oxoecdysteroid as intermediate. Copyright © 1989 Wiley‐Liss, Inc.
Note:
Related Files :
3α‐hydroxyecdysteroids
3‐dehydroecdysone
3‐epiecdysone
3‐epimerization
molting hormone inactivation
NADH
Show More
Related Content
More details
DOI :
10.1002/arch.940120402
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28588
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
Scientific Publication
Ecdysone oxidase and 3‐oxoecdysteroid reductases in Manduca sexta midgut: Kinetic parameters
12
Weirich, G.F., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Thompson, M.J., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Svoboda, J.A., Insect Hormone Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, United States
Ecdysone oxidase and 3‐oxoecdysteroid reductases in Manduca sexta midgut: Kinetic parameters
Ecdysone and 20‐hydroxyecdysone are converted to their 3‐epimers by enzymes in the midgut cytosol of Manduca sexta larvae. A partially purified cytosol preparation has been used to analyze the nature of and the interaction between these enzymes. The cytosol was shown to contain ecdysone oxidase, one or more 3‐oxoecdysteroid 3α‐reductase(s), and one or more 3‐oxoecdysteroid 3β‐reductase(s). The reductases reacted at different velocities with NADH and NADPH. With NADH, 3α‐reduction was the major reaction; with NADPH, 3β‐reduction was the major reaction. The apparent kinetic parameters for the enzymes support the assumed two‐step mechanism for the 3‐epimerization with a 3‐oxoecdysteroid as intermediate. Copyright © 1989 Wiley‐Liss, Inc.
Scientific Publication
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