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Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression
Year:
2015
Source of publication :
Frontiers in Microbiology
Authors :
Shemesh, Moshe
;
.
Volume :
6
Co-Authors:
Oknin, H., Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Biofilm Research Laboratory, Faculty of Dental Medicine, Institute of Dental Sciences, Hebrew University-Hadassah, Jerusalem, Israel
Steinberg, D., Biofilm Research Laboratory, Faculty of Dental Medicine, Institute of Dental Sciences, Hebrew University-Hadassah, Jerusalem, Israel
Shemesh, M., Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
The objective of this study was to investigate the effect of Mg2+ ions on biofilm formation by Bacillus species, which are considered as problematic microorganisms in the food industry. We found that magnesium ions are capable to inhibit significantly biofilm formation of Bacillus species at 50 mM concentration and higher. We further report that Mg2+ ions don't inhibit bacterial growth at elevated concentrations; hence, the mode of action of Mg2+ ions is apparently specific to inhibition of biofilm formation. Biofilm formation depends on the synthesis of extracellular matrix, whose production in Bacillus subtilis is specified by two major operons: the epsA-O and tapA operons. We analyzed the effect of Mg2+ ions on matrix gene expression using transcriptional fusions of the promoters for eps and tapA to the gene encoding β galactosidase. The expression of the two matrix operons was reduced drastically in response to Mg2+ ions suggesting about their inhibitory effect on expression of the matrix genes in B. subtilis. Since the matrix gene expression is tightly controlled by Spo0A dependent pathway, we conclude that Mg2+ ions could affect the signal transduction for biofilm formation through this pathway. © 2015 Oknin, Steinberg and Shemesh.
Note:
Related Files :
animal cell
Bacillus
confocal laser microscopy
gene expression
Magnesium ions
Microbial development
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More details
DOI :
10.3389/fmicb.2015.00907
Article number:
907
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28607
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
Scientific Publication
Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression
6
Oknin, H., Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel, Biofilm Research Laboratory, Faculty of Dental Medicine, Institute of Dental Sciences, Hebrew University-Hadassah, Jerusalem, Israel
Steinberg, D., Biofilm Research Laboratory, Faculty of Dental Medicine, Institute of Dental Sciences, Hebrew University-Hadassah, Jerusalem, Israel
Shemesh, M., Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel
Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression
The objective of this study was to investigate the effect of Mg2+ ions on biofilm formation by Bacillus species, which are considered as problematic microorganisms in the food industry. We found that magnesium ions are capable to inhibit significantly biofilm formation of Bacillus species at 50 mM concentration and higher. We further report that Mg2+ ions don't inhibit bacterial growth at elevated concentrations; hence, the mode of action of Mg2+ ions is apparently specific to inhibition of biofilm formation. Biofilm formation depends on the synthesis of extracellular matrix, whose production in Bacillus subtilis is specified by two major operons: the epsA-O and tapA operons. We analyzed the effect of Mg2+ ions on matrix gene expression using transcriptional fusions of the promoters for eps and tapA to the gene encoding β galactosidase. The expression of the two matrix operons was reduced drastically in response to Mg2+ ions suggesting about their inhibitory effect on expression of the matrix genes in B. subtilis. Since the matrix gene expression is tightly controlled by Spo0A dependent pathway, we conclude that Mg2+ ions could affect the signal transduction for biofilm formation through this pathway. © 2015 Oknin, Steinberg and Shemesh.
Scientific Publication
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