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Expression of the nopaline synthase gene in Escherichia coll
Year:
1985
Source of publication :
Gene
Authors :
Gafni, Yedidya
;
.
Volume :
39
Co-Authors:
Gafni, Y., CIBA-Geigy Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, United States
Chilton, M.-D., CIBA-Geigy Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, United States
Facilitators :
From page:
141
To page:
146
(
Total pages:
6
)
Abstract:
The Agrobacterium tumor-inducing (Ti) plasmid pTiT37 encodes nopaline synthase (NOS) gene (nos) with eukaryotic promoter elements that is expressed in transformed plant cells but not in the bacterial host. We have fused the nos gene to the Escherichia coli trp promoter, and observed synthesis of NOS in E. coli. The nopaline produced by this enzyme is excreted into the culture medium. NOS is enzymatically active at 30 °C but not 37 °C, as based on nopaline production. NOS protein is produced at both temperatures, based on production in minicells. © 1985.
Note:
Related Files :
Agrobacterium
gene expression
genetic engineering
Heredity
temperature
Show More
Related Content
More details
DOI :
10.1016/0378-1119(85)90307-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28615
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
Scientific Publication
Expression of the nopaline synthase gene in Escherichia coll
39
Gafni, Y., CIBA-Geigy Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, United States
Chilton, M.-D., CIBA-Geigy Biotechnology Research, P.O. Box 12257, Research Triangle Park, NC 27709, United States
Expression of the nopaline synthase gene in Escherichia coll
The Agrobacterium tumor-inducing (Ti) plasmid pTiT37 encodes nopaline synthase (NOS) gene (nos) with eukaryotic promoter elements that is expressed in transformed plant cells but not in the bacterial host. We have fused the nos gene to the Escherichia coli trp promoter, and observed synthesis of NOS in E. coli. The nopaline produced by this enzyme is excreted into the culture medium. NOS is enzymatically active at 30 °C but not 37 °C, as based on nopaline production. NOS protein is produced at both temperatures, based on production in minicells. © 1985.
Scientific Publication
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