נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
A comparative study of the involvement of 17 arabidopsis myosin family members on the motility of golgi and other organelles
Year:
2009
Source of publication :
Plant physiology (source)
Authors :
Abu-Abied, Mohamad
;
.
Avisar, Dror
;
.
Belausov, Eduard
;
.
Sadot, Einat
;
.
Volume :
150
Co-Authors:
Avisar, D., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Abu-Abied, M., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Sadott, E., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Hawes, C., School of Life Sciences, Oxford Brookes University, Oxford OX3 OBP, United Kingdom
Sparkes, I.A., School of Life Sciences, Oxford Brookes University, Oxford OX3 OBP, United Kingdom
Facilitators :
From page:
700
To page:
709
(
Total pages:
10
)
Abstract:
Gene families with multiple members are predicted to have individuals with overlapping functions. We examined all of the Arabidopsis (Arabidopsis thaliana) myosin family members for their involvement in Golgi and other organelle motility. Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ tail or tail domains only were fused to fluorescent markers and coexpressed with a Golgi marker in two different plants. We tracked and calculated Golgi body displacement rate in the presence of all myosin truncations and found that tail fragments of myosins MYAl, MYA2, XI-C, XI-E, XI-I, and XI-K were the best inhibitors of Golgi body movement in the two plants. Tail fragments of myosins XI-B, XI-F, XI-H, and ATM1 had an inhibitory effect on Golgi bodies only in Nicotiana tabacum, while tail fragments of myosins XI-G and ATM2 had a slight effect on Golgi body motility only in Nicotiana benthamiana. The best myosin inhibitors of Golgi body motility were able to arrest mitochondrial movement too. No exclusive colocalization was found between these myosins and Golgi bodies in our system, although the excess of cytosolic signal observed could mask myosin molecules bound to the surface of the organelle. From the preserved actin filaments found in the presence of enhanced green fluorescent protein fusions of truncated myosins and the motility of myosin punctae, we conclude that global arrest of actomyosin-derived cytoplasmic streaming had not occurred. Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells. © 2009 American Society of Plant Biologists.
Note:
Related Files :
arabidopsis
Arabidopsis thaliana
chemistry
Golgi complex
metabolism
multigene family
myosin
Peptide Fragments
photoprotein
Show More
Related Content
More details
DOI :
10.1104/pp.109.136853
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28645
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
You may also be interested in
Scientific Publication
A comparative study of the involvement of 17 arabidopsis myosin family members on the motility of golgi and other organelles
150
Avisar, D., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Abu-Abied, M., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Sadott, E., Institute of Plant Sciences, Volcani Center, Bet-Dagan 50250, Israel
Hawes, C., School of Life Sciences, Oxford Brookes University, Oxford OX3 OBP, United Kingdom
Sparkes, I.A., School of Life Sciences, Oxford Brookes University, Oxford OX3 OBP, United Kingdom
A comparative study of the involvement of 17 arabidopsis myosin family members on the motility of golgi and other organelles
Gene families with multiple members are predicted to have individuals with overlapping functions. We examined all of the Arabidopsis (Arabidopsis thaliana) myosin family members for their involvement in Golgi and other organelle motility. Truncated fragments of all 17 annotated Arabidopsis myosins containing either the IQ tail or tail domains only were fused to fluorescent markers and coexpressed with a Golgi marker in two different plants. We tracked and calculated Golgi body displacement rate in the presence of all myosin truncations and found that tail fragments of myosins MYAl, MYA2, XI-C, XI-E, XI-I, and XI-K were the best inhibitors of Golgi body movement in the two plants. Tail fragments of myosins XI-B, XI-F, XI-H, and ATM1 had an inhibitory effect on Golgi bodies only in Nicotiana tabacum, while tail fragments of myosins XI-G and ATM2 had a slight effect on Golgi body motility only in Nicotiana benthamiana. The best myosin inhibitors of Golgi body motility were able to arrest mitochondrial movement too. No exclusive colocalization was found between these myosins and Golgi bodies in our system, although the excess of cytosolic signal observed could mask myosin molecules bound to the surface of the organelle. From the preserved actin filaments found in the presence of enhanced green fluorescent protein fusions of truncated myosins and the motility of myosin punctae, we conclude that global arrest of actomyosin-derived cytoplasmic streaming had not occurred. Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells. © 2009 American Society of Plant Biologists.
Scientific Publication
You may also be interested in