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Hyperinsulinemic clamp modulates milk fat globule lipid composition in goats
Year:
2012
Source of publication :
Journal of Dairy Science
Authors :
Mabjeesh, Sameer Jermaya
;
.
Shamay, Avi
;
.
Volume :
95
Co-Authors:
Argov-Argaman, N., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Mbogori, T., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Sabastian, C., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Shamay, A., Animal Science Department, The Volcani Center, The Ministry of Agriculture, POB 6, Bet Dagan, Israel
Mabjeesh, S.J., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Facilitators :
From page:
5776
To page:
5787
(
Total pages:
12
)
Abstract:
We determined the effect of insulin on milk fatty acid (FA) and lipid composition in goats. Four dairy goats, 150. d in milk, were subjected to hyperinsulinemic clamp (treatment) or saline (control) infusion for 4. d in a crossover design study. Composition and concentration of plasma and milk FA, triglycerides, phospholipids, sphingolipids, and cholesterol were determined. Mammary gland biopsies were taken at the end of each experimental period and lipogenic gene expression was determined. Plasma insulin was elevated 3.5-fold, whereas plasma glucose remained constant during the treatment period. Feed intake decreased by 26% and fat yield decreased by 17% relative to controls. No change in nonesterified FA concentration was found between controls and treatment. Compared with controls, insulin decreased yield of long-chain saturated FA by 14%. Milk concentration of long-chain FA was reduced by 3%, whereas that of medium-chain FA increased by 5% during the treatment compared with controls. Hyperinsulinemic clamps increased the yields of milk phospholipids by 9% and cholesterol by 16%, whereas it only tended to decrease triglyceride yields (by 11%). Hyperinsulinemic treatment resulted in compositional changes in the milk fat globule membrane, as reflected by 15 and 9% decreases in phosphatidylethanolamine and phosphatidylcholine concentrations, respectively. Lipogenic gene expression of acyl coenzyme A carboxylase, stearoyl coenzyme A desaturase, and FA synthase did not change, whereas lipoprotein lipase gene expression tended toward an increase in the treatment period compared with controls. Hyperinsulinemic clamps reduce the availability of long-chain FA, which are considered to originate from the diet and adipose lipolysis for milk lipid synthesis by the mammary gland of goats. Under these conditions, long-chain FA might be preferentially channeled to phospholipid rather than triglyceride synthesis, hence increasing phospholipid yields. Mechanisms determining FA distribution among milk lipid components and the consequences of altered milk fat globule membrane lipid composition remained to be elucidated. © 2012 American Dairy Science Association.
Note:
Related Files :
Animal
Animals
Blood
fatty acids
Female
glycolipid
goats
Lipids
milk
phospholipid
Show More
Related Content
More details
DOI :
10.3168/jds.2012-5569
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28666
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
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Scientific Publication
Hyperinsulinemic clamp modulates milk fat globule lipid composition in goats
95
Argov-Argaman, N., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Mbogori, T., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Sabastian, C., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Shamay, A., Animal Science Department, The Volcani Center, The Ministry of Agriculture, POB 6, Bet Dagan, Israel
Mabjeesh, S.J., Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, The Hebrew University of Jerusalem, POB 12, Jerusalem, Israel
Hyperinsulinemic clamp modulates milk fat globule lipid composition in goats
We determined the effect of insulin on milk fatty acid (FA) and lipid composition in goats. Four dairy goats, 150. d in milk, were subjected to hyperinsulinemic clamp (treatment) or saline (control) infusion for 4. d in a crossover design study. Composition and concentration of plasma and milk FA, triglycerides, phospholipids, sphingolipids, and cholesterol were determined. Mammary gland biopsies were taken at the end of each experimental period and lipogenic gene expression was determined. Plasma insulin was elevated 3.5-fold, whereas plasma glucose remained constant during the treatment period. Feed intake decreased by 26% and fat yield decreased by 17% relative to controls. No change in nonesterified FA concentration was found between controls and treatment. Compared with controls, insulin decreased yield of long-chain saturated FA by 14%. Milk concentration of long-chain FA was reduced by 3%, whereas that of medium-chain FA increased by 5% during the treatment compared with controls. Hyperinsulinemic clamps increased the yields of milk phospholipids by 9% and cholesterol by 16%, whereas it only tended to decrease triglyceride yields (by 11%). Hyperinsulinemic treatment resulted in compositional changes in the milk fat globule membrane, as reflected by 15 and 9% decreases in phosphatidylethanolamine and phosphatidylcholine concentrations, respectively. Lipogenic gene expression of acyl coenzyme A carboxylase, stearoyl coenzyme A desaturase, and FA synthase did not change, whereas lipoprotein lipase gene expression tended toward an increase in the treatment period compared with controls. Hyperinsulinemic clamps reduce the availability of long-chain FA, which are considered to originate from the diet and adipose lipolysis for milk lipid synthesis by the mammary gland of goats. Under these conditions, long-chain FA might be preferentially channeled to phospholipid rather than triglyceride synthesis, hence increasing phospholipid yields. Mechanisms determining FA distribution among milk lipid components and the consequences of altered milk fat globule membrane lipid composition remained to be elucidated. © 2012 American Dairy Science Association.
Scientific Publication
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