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Isolation and identification of 26‐hydroxyecdysone 2‐phosphate: An ecdysteroid conjugate of eggs and ovaries of the tobacco hornworm, Manduca sexta
Year:
1987
Authors :
Svoboda, James
;
.
Volume :
4
Co-Authors:
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Rees, H.H., Department of Biochemistry, University of Liverpool, Liverpool, United Kingdom
Wilzer, K.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Facilitators :
From page:
183
To page:
190
(
Total pages:
8
)
Abstract:
Maturing eggs (48 to 64 h old) of the tobacco hornworm, Manduca sexta, contain at least three ecdysteroid conjugates, two of which have been previously identified as 26‐hydroxyecdysone 26‐phosphate (the major conjugate) and 26‐hydroxyecdysone 22‐glucoside. In this study we have isolated and identified the third conjugate as 26‐hydroxyecdysone 2‐phosphate by XAD‐2 chromatography, C18 SEP‐PAK separation, ion suppression reversed‐phase high‐performance liquid chromatography, nuclear magnetic resonance, and fast atom bombardment mass spectrometry. This compound is the second most abundant conjugate of ovaries from 4‐day‐old adult females. The possible role for this ecdysteroid conjugate is discussed. Copyright © 1987 Wiley‐Liss, Inc.
Note:
Related Files :
26‐hydroxyecdysone 2‐phosphate
[14C]cholesterol
enzymatic hydrolysis
radiolabeled ecdysteroid conjugates
Show More
Related Content
More details
DOI :
10.1002/arch.940040304
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28667
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:40
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Scientific Publication
Isolation and identification of 26‐hydroxyecdysone 2‐phosphate: An ecdysteroid conjugate of eggs and ovaries of the tobacco hornworm, Manduca sexta
4
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Rees, H.H., Department of Biochemistry, University of Liverpool, Liverpool, United Kingdom
Wilzer, K.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Isolation and identification of 26‐hydroxyecdysone 2‐phosphate: An ecdysteroid conjugate of eggs and ovaries of the tobacco hornworm, Manduca sexta
Maturing eggs (48 to 64 h old) of the tobacco hornworm, Manduca sexta, contain at least three ecdysteroid conjugates, two of which have been previously identified as 26‐hydroxyecdysone 26‐phosphate (the major conjugate) and 26‐hydroxyecdysone 22‐glucoside. In this study we have isolated and identified the third conjugate as 26‐hydroxyecdysone 2‐phosphate by XAD‐2 chromatography, C18 SEP‐PAK separation, ion suppression reversed‐phase high‐performance liquid chromatography, nuclear magnetic resonance, and fast atom bombardment mass spectrometry. This compound is the second most abundant conjugate of ovaries from 4‐day‐old adult females. The possible role for this ecdysteroid conjugate is discussed. Copyright © 1987 Wiley‐Liss, Inc.
Scientific Publication
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