Co-Authors:
Kumar, M., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Pandya-Kumar, N., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Dam, A., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Haor, H., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Mayzlish-Gati, E., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Wininger, S., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Abu-Abied, M., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
McErlean, C.S.P., School of Chemistry, University of SydneyNSW, Australia
Bromhead, L.J., School of Chemistry, University of SydneyNSW, Australia
Prandi, C., Dipartimento di Chimica, Turin University, Torino, Italy
Kapulnik, Y., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Koltai, H., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, Israel
Abstract:
Strigolactones (SLs) are plant hormones that regulate the plant response to phosphate (Pi) growth conditions. At least part of SL-signalling execution in roots involves MAX2-dependent effects on PIN2 polar localization in the plasma membrane (PM) and actin bundling and dynamics. We examined PIN2 expression, PIN2 PM localization, endosome trafficking, and actin bundling under low-Pi conditions: a MAX2-dependent reduction in PIN2 trafficking and polarization in the PM, reduced endosome trafficking, and increased actin-filament bundling were detected in root cells. The intracellular protein trafficking that is related to PIN proteins but unassociated with AUX1 PM localization was selectively inhibited. Exogenous supplementation of the synthetic SL GR24 to a SL-deficient mutant (max4) led to depletion of PIN2 from the PM under low-Pi conditions. Accordingly, roots of mutants in MAX2, MAX4, PIN2, TIR3, and ACTIN2 showed a reduced low-Pi response compared with the wild type, which could be restored by auxin (for all mutants) or GR24 (for all mutants except max2-1). Changes in PIN2 polarity, actin bundling, and vesicle trafficking may be involved in the response to low Pi in roots, dependent on SL/MAX2 signalling. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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