נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Involvement of two different urf-s related mitochondrial sequences in the molecular evolution of the CMS-specific S-Pcf locus in petunia
Year:
1995
Source of publication :
MGG Molecular & General Genetics
Authors :
Firon, Nurit
;
.
Gidoni, David
;
.
Izhar, Shamay
;
.
Tabib, Yona
;
.
Volume :
248
Co-Authors:
Yesodi, V., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Gidoni, D., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Tabib, Y., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Firon, N., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Facilitators :
From page:
540
To page:
546
(
Total pages:
7
)
Abstract:
In petunia, a mitochondrial (mt) locus, S-Pcf, has been found to be strongly associated with cytoplasmic male sterility (CMS). The S-Pcf locus consists of three open reading frames (ORF) that are co-transcribed. The first ORF, Pcf, contains parts of the atp9 and coxII genes and an unidentified reading frame, urf-s. The second and third ORFs contain NADH dehydrogenase subunit 3 (nad3) and ribosomal protein S12 (rps12) sequences, respectively. The nad3 and rps12 sequences included in the S-Pcf locus are identical to the corresponding sequences on the mt genome of fertile petunia. In both CMS and fertile petunia, only a single copy of nad3 and rps12 has been detected on the physical map of the main mt genome. The origin of the urf-s sequence and the molecular events leading to the formation of the chimeric S-Pcf locus are not known. This paper presents evidence indicating that two different mt sequences, related to urf-s and found in fertile petunia lines (orf-h and Rf-1), might have been involved in the molecular evolution of the S-Pcf locus. Southern analysis of mtDNA derived from both fertile and sterile petunia plants suggests that one of these urf-s related sequences (showing 100% homology to urf-s and termed orf-h) is located on a sublimon. An additional, low-homology urf-s related sequence (Rf-1) is shown to be located on the main mt genome 5′ to the nad3 gene. It is, thus, suggested that the sequence of events leading to the generation of the S-Pcf locus might have involved introduction of the orf-h sequence, via homologous recombination, into the main mt genome 5′ to nad3 at the region where the Rf-1 sequence is located. © 1995 Springer-Verlag.
Note:
Related Files :
CMS
Evolution
gene locus
gene structure
mitochondrial DNA
Petunia
plant
Plants
sequence analysis
Show More
Related Content
More details
DOI :
10.1007/BF02423449
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28706
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:41
You may also be interested in
Scientific Publication
Involvement of two different urf-s related mitochondrial sequences in the molecular evolution of the CMS-specific S-Pcf locus in petunia
248
Yesodi, V., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Gidoni, D., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Tabib, Y., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Firon, N., Department of Plant Genetics, The Volcani Center, Bet Dagan, 50 250, Israel
Involvement of two different urf-s related mitochondrial sequences in the molecular evolution of the CMS-specific S-Pcf locus in petunia
In petunia, a mitochondrial (mt) locus, S-Pcf, has been found to be strongly associated with cytoplasmic male sterility (CMS). The S-Pcf locus consists of three open reading frames (ORF) that are co-transcribed. The first ORF, Pcf, contains parts of the atp9 and coxII genes and an unidentified reading frame, urf-s. The second and third ORFs contain NADH dehydrogenase subunit 3 (nad3) and ribosomal protein S12 (rps12) sequences, respectively. The nad3 and rps12 sequences included in the S-Pcf locus are identical to the corresponding sequences on the mt genome of fertile petunia. In both CMS and fertile petunia, only a single copy of nad3 and rps12 has been detected on the physical map of the main mt genome. The origin of the urf-s sequence and the molecular events leading to the formation of the chimeric S-Pcf locus are not known. This paper presents evidence indicating that two different mt sequences, related to urf-s and found in fertile petunia lines (orf-h and Rf-1), might have been involved in the molecular evolution of the S-Pcf locus. Southern analysis of mtDNA derived from both fertile and sterile petunia plants suggests that one of these urf-s related sequences (showing 100% homology to urf-s and termed orf-h) is located on a sublimon. An additional, low-homology urf-s related sequence (Rf-1) is shown to be located on the main mt genome 5′ to the nad3 gene. It is, thus, suggested that the sequence of events leading to the generation of the S-Pcf locus might have involved introduction of the orf-h sequence, via homologous recombination, into the main mt genome 5′ to nad3 at the region where the Rf-1 sequence is located. © 1995 Springer-Verlag.
Scientific Publication
You may also be interested in