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Pinner, E., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Carmel, O., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Bercovier, H., Department of Bacteriology, Hadassah Medical School, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Sela, S., Department of Bacteriology, Hadassah Medical School, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Padan, E., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Schuldiner, S., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Na+/H+ antiporter activity is wide-spread and plays essential physiological roles. We found that several Enterobacteriaceae share conserved sequences with nhaA, the gene coding for an E. coli antiporter. A ΔnhaA strain which is sensitive to Na+ and Li+, was used to clone by complementation a DNA fragment from Salmonella enteritidis which confers resistance to the ions. The cloned fragment increased Na+/H+ antiport activity in membranes isolated from strains carrying the respective hybrid plasmid. DNA sequence analysis of the insert revealed two open reading frames. Both encode putative polypeptides which are closely homologous to the nhaA and nhaR gene products from Escherichia coli. The antiporter activity displays properties very similar to that of the E. coli NhaA, namely, it is activiated by alkaline pH and recognizes Li+ with high affinity. © 1992 Springer-Verlag.
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Cloning, sequencing and expression of the nhaA and nhaR genes from Salmonella entiritidis
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Pinner, E., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Carmel, O., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Bercovier, H., Department of Bacteriology, Hadassah Medical School, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Sela, S., Department of Bacteriology, Hadassah Medical School, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Padan, E., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Schuldiner, S., Department of Molecular and Microbial Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jersusalem, 91 904, Israel
Cloning, sequencing and expression of the nhaA and nhaR genes from Salmonella entiritidis
Na+/H+ antiporter activity is wide-spread and plays essential physiological roles. We found that several Enterobacteriaceae share conserved sequences with nhaA, the gene coding for an E. coli antiporter. A ΔnhaA strain which is sensitive to Na+ and Li+, was used to clone by complementation a DNA fragment from Salmonella enteritidis which confers resistance to the ions. The cloned fragment increased Na+/H+ antiport activity in membranes isolated from strains carrying the respective hybrid plasmid. DNA sequence analysis of the insert revealed two open reading frames. Both encode putative polypeptides which are closely homologous to the nhaA and nhaR gene products from Escherichia coli. The antiporter activity displays properties very similar to that of the E. coli NhaA, namely, it is activiated by alkaline pH and recognizes Li+ with high affinity. © 1992 Springer-Verlag.
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