אסיף מאגר המחקר החקלאי

Parathion degradation by a Pseudomonas sp. and a Xanthomonas sp. and by their crude enzyme extracts as affected by some cations

Year:

1993

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Volume :

25

Co-Authors:

Tchelet, R., Department of Molecular Microbiology and Biotechnology, Tel Aviv University, George S. Wise Faculty of Life Sciences, Ramat Aviv, 69978 Tel Aviv, Israel
Levanon, D., IGAL-Galilee Technological Center, 10200 Kiryat Shmona, Israel
Mingelgrin, U., Institute of Soils and Water, ARO, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Henis, Y., Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel

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Abstract:

A Pseudomonas sp. and a Xanthomonas sp. were isolated from a pesticide disposal site in northern Israel. Both degraded parathion in two stages. In the first stage, p-nitrophenol (PNP) was released by a parathion hydrolase, while in the second stage PNP was degraded. The effect of Cu2+, Fe2+, Ca2+, Mn2+, Al3+, Zn2+ and NaEDTA on parathion hydrolysis to PNP by growing cultures and by crude enzyme extracts of the isolates was studied. Parathion hydrolysis by both either living cells or crude enzyme extracts of the Pseudomonas sp. was inhibited by Cu2+ even at a concentration of 20 μmoll-1, as opposed to this cation's stimulatory effect on the degradation of parathion by the Xanthomonas sp. Other cations investigated had a smaller effect on the rate of degradation by either bacterium. NaEDTA strongly inhibited parathion hydrolysis in the living cells, but not in the crude enzyme extracts of the Pseudomonas sp. In contrast, NaEDTA inhibited parathion hydrolysis in either living cells or crude enzyme extracts of the Xanthomonas sp. © 1993.

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