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Plant and Cell Physiology
Rosner, A., Weizmann Institute of Science, Rehovot, Israel
Posner, H.B., Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Weizmann Institute of Science, Rehovot, Israel
The synthesis and processing of rapidly labelled RNAs from Lemna perpusilla 6746 was followed by polyacrylamide electrophoresis, as the first step in a study of the changes which occur with environmental changes. Two RNAs with high apparent molecular weights of 2.8 M and 2.3 M were detected. Time-course study results were consistent with the idea that the 2.8 M was cleaved to form 2.3 M RNA and "excess" RNA with a molecular weight of 0.5 M. The latter had a far lower turnover than the 2.3 M RNA. Another rapidly labelled RNA which has not been described before in plants, had an apparent weight of 1.2 M. This was followed by labelling the 1.4 M component, then the light rRNA (0.7 M), and finally the heavy rRNA (1.3 M). A relatively large amount of high molecular weight, polydisperse RNA was synthesized under steadystate conditions.Incubation of plants on distilled water reduced synthesis of 1.2 M RNA, while other components were less affected. The processing of rRNA precursors was also slowed. © 1973 Oxford University Press.
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Synthesis and processing of RNA in lemna: Characterization by gel electrophoresis
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Rosner, A., Weizmann Institute of Science, Rehovot, Israel
Posner, H.B., Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Weizmann Institute of Science, Rehovot, Israel
Synthesis and processing of RNA in lemna: Characterization by gel electrophoresis
The synthesis and processing of rapidly labelled RNAs from Lemna perpusilla 6746 was followed by polyacrylamide electrophoresis, as the first step in a study of the changes which occur with environmental changes. Two RNAs with high apparent molecular weights of 2.8 M and 2.3 M were detected. Time-course study results were consistent with the idea that the 2.8 M was cleaved to form 2.3 M RNA and "excess" RNA with a molecular weight of 0.5 M. The latter had a far lower turnover than the 2.3 M RNA. Another rapidly labelled RNA which has not been described before in plants, had an apparent weight of 1.2 M. This was followed by labelling the 1.4 M component, then the light rRNA (0.7 M), and finally the heavy rRNA (1.3 M). A relatively large amount of high molecular weight, polydisperse RNA was synthesized under steadystate conditions.Incubation of plants on distilled water reduced synthesis of 1.2 M RNA, while other components were less affected. The processing of rRNA precursors was also slowed. © 1973 Oxford University Press.
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