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In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands
Year:
1989
Source of publication :
Insect Biochemistry
Authors :
Rafaeli, Ada
;
.
Soroker, Victoria
;
.
Volume :
19
Co-Authors:
Soroker, V., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Rafaeli, A., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Facilitators :
From page:
1
To page:
5
(
Total pages:
5
)
Abstract:
Isolated pheromone gland preparations of Heliothis armigera incorporateradioactivity from [14C]sodium acetate at a linear rate for 24 h when incubated in a physiological saline. This incorporation is stimulated when methanolic or partially purified brain-complex extracts are present in the incubation medium and the stimulation is dose-dependent. The radioactive products extracted from the pheromone glands and media by hexane, revealed incorporation of radioactivity into a product exhibiting the same mobility as (Z)-11 hexadecenal, the main component of the pheromone of H. armigera, as analyzed by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). In addition, gas chromatographic (GC) analysis of the hexane soluble products after incubation in the absence of [14C]sodium acetate revealed a significant stimulation of the concentration of (Z)-11 hexadecenal by brain-complex extracts. © 1989.
Note:
Related Files :
[14C]acetate incorporation
Heliothis armigera
hormone
in vitro pheromone glands
pheromone
Show More
Related Content
More details
DOI :
10.1016/0020-1790(89)90002-4
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28813
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
You may also be interested in
Scientific Publication
In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands
19
Soroker, V., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Rafaeli, A., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands
Isolated pheromone gland preparations of Heliothis armigera incorporateradioactivity from [14C]sodium acetate at a linear rate for 24 h when incubated in a physiological saline. This incorporation is stimulated when methanolic or partially purified brain-complex extracts are present in the incubation medium and the stimulation is dose-dependent. The radioactive products extracted from the pheromone glands and media by hexane, revealed incorporation of radioactivity into a product exhibiting the same mobility as (Z)-11 hexadecenal, the main component of the pheromone of H. armigera, as analyzed by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). In addition, gas chromatographic (GC) analysis of the hexane soluble products after incubation in the absence of [14C]sodium acetate revealed a significant stimulation of the concentration of (Z)-11 hexadecenal by brain-complex extracts. © 1989.
Scientific Publication
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