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Insect Biochemistry
Soroker, V., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Rafaeli, A., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Isolated pheromone gland preparations of Heliothis armigera incorporateradioactivity from [14C]sodium acetate at a linear rate for 24 h when incubated in a physiological saline. This incorporation is stimulated when methanolic or partially purified brain-complex extracts are present in the incubation medium and the stimulation is dose-dependent. The radioactive products extracted from the pheromone glands and media by hexane, revealed incorporation of radioactivity into a product exhibiting the same mobility as (Z)-11 hexadecenal, the main component of the pheromone of H. armigera, as analyzed by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). In addition, gas chromatographic (GC) analysis of the hexane soluble products after incubation in the absence of [14C]sodium acetate revealed a significant stimulation of the concentration of (Z)-11 hexadecenal by brain-complex extracts. © 1989.
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In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands
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Soroker, V., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
Rafaeli, A., Department of Entomology, Faculty of Agriculture, Rehovot 76-100, Israel
In vitro hormonal stimulation of [14C]acetate incorporation by Heliothis armigera pheromone glands
Isolated pheromone gland preparations of Heliothis armigera incorporateradioactivity from [14C]sodium acetate at a linear rate for 24 h when incubated in a physiological saline. This incorporation is stimulated when methanolic or partially purified brain-complex extracts are present in the incubation medium and the stimulation is dose-dependent. The radioactive products extracted from the pheromone glands and media by hexane, revealed incorporation of radioactivity into a product exhibiting the same mobility as (Z)-11 hexadecenal, the main component of the pheromone of H. armigera, as analyzed by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). In addition, gas chromatographic (GC) analysis of the hexane soluble products after incubation in the absence of [14C]sodium acetate revealed a significant stimulation of the concentration of (Z)-11 hexadecenal by brain-complex extracts. © 1989.
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