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Uptake and fate of IAA in apple callus tissues: Metabolism of IAA-2-14C
Year:
1975
Source of publication :
Plant and Cell Physiology
Authors :
Epstein, Ephraim
;
.
Klein, Isaac
;
.
Lavee, Shimon
;
.
Volume :
16
Co-Authors:
Facilitators :
From page:
305
To page:
311
(
Total pages:
7
)
Abstract:
Young and old apple callus tissues were incubated in light or darkness with IAA-2-14C. A large portion of the IAA disappeared from the medium with both young and old calluses. Whereas with old calluses the loss was mainly due to IAA destruction, young calluses accumulated IAA to a level which exceeded the external concentration and, in addition, seemed to protect it from breakdown. After 24 hr the level of IAA-2-14C in the medium dropped to 50% with old calluses both in the dark and light, and with young calluses to 20% in the light and 50% in the dark. Chromatography and scanning of the media and calluses showed that IAA was converted into two compounds (comp. A and comp. B). The amounts and proportions of these metabolites in the medium and tissue were dependent on the different treatments and callus age. The breakdown of IAA by old tissue gave rise to a higher level of comp. B both in the tissue and medium, particularly after 6 hr of incubation. In the medium of young tissues the level of comp. A was higher than comp. B while equal amounts of the two compounds were detected in the tissue, itself. The origin of the IAA products in the tissue was probably endogenous and not via absorption from the medium. The IAA metabolism of apple callus tissues seems to proceed via the oxindole pathway and it is proposed that compounds A and B are 3-hydroxymethyloxindole and 3-methylene oxindole, respectively. © 1975 Oxford University Press.
Note:
Related Files :
Apple
IAA
Malus sylvestris
metabolism
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Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28848
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
Scientific Publication
Uptake and fate of IAA in apple callus tissues: Metabolism of IAA-2-14C
16
Uptake and fate of IAA in apple callus tissues: Metabolism of IAA-2-14C
Young and old apple callus tissues were incubated in light or darkness with IAA-2-14C. A large portion of the IAA disappeared from the medium with both young and old calluses. Whereas with old calluses the loss was mainly due to IAA destruction, young calluses accumulated IAA to a level which exceeded the external concentration and, in addition, seemed to protect it from breakdown. After 24 hr the level of IAA-2-14C in the medium dropped to 50% with old calluses both in the dark and light, and with young calluses to 20% in the light and 50% in the dark. Chromatography and scanning of the media and calluses showed that IAA was converted into two compounds (comp. A and comp. B). The amounts and proportions of these metabolites in the medium and tissue were dependent on the different treatments and callus age. The breakdown of IAA by old tissue gave rise to a higher level of comp. B both in the tissue and medium, particularly after 6 hr of incubation. In the medium of young tissues the level of comp. A was higher than comp. B while equal amounts of the two compounds were detected in the tissue, itself. The origin of the IAA products in the tissue was probably endogenous and not via absorption from the medium. The IAA metabolism of apple callus tissues seems to proceed via the oxindole pathway and it is proposed that compounds A and B are 3-hydroxymethyloxindole and 3-methylene oxindole, respectively. © 1975 Oxford University Press.
Scientific Publication
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