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A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants
Year:
1999
Source of publication :
Plant Journal
Authors :
Arazi, Tzahi
;
.
Volume :
20
Co-Authors:
Arazi, T., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Sunkar, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Kaplan, B., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Fromm, H., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Centre for Plant Sciences, Leeds Inst. of Biotech. and Agric., University of Leeds, Leeds LS2 9JT, United Kingdom
Facilitators :
From page:
171
To page:
182
(
Total pages:
12
)
Abstract:
All organisms require a minimal amount of metal ions to sustain their metabolism, growth and development. At the same time, their intrinsic metal-uptake systems render them vulnerable to toxic levels of metals in the biosphere. Using radiolabeled recombinant calmodulin as a probe to screen a tobacco cDNA library, we have discovered a protein designated NtCBP4 (Nicotiana tabacum calmodulin-binding protein) that can modulate plant tolerance to heavy metals. Structurally, NtCBP4 is similar to vertebrate and invertebrate K+ and to nonselective cation channels, as well as to recently reported proteins from barley and Arabidopsis. Here we report on the subcellular localization of NtCBP4 and the phenotype of transgenic plants overexpressing this protein. The localization of NtCBP4 in the plasma membrane was manifested by fractionating tobacco membranes on sucrose gradients or by aqueous two-phase partitioning, and subsequently using immunodetection. Several independent transgenic lines expressing NtCBP4 had higher than normal levels of NtCBP4. These transgenic lines were indistinguishable from wild type under normal growth conditions. However, they exhibited improved tolerance to Ni2+ and hypersensitivity to Pb2+, which are associated with reduced Ni2+ accumulation and enhanced Pb2+ accumulation, respectively. To our knowledge this is the first report of a plant protein that modulates plant tolerance or accumulation of Pb2+. We propose that NtCBP4 is involved in metal uptake across the plant plasma membrane. This gene may prove useful for implementing selective ion tolerance in crops and improving phytoremediation strategies.
Note:
Related Files :
arabidopsis
cell membrane
nickel
phenotype
plant development
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More details
DOI :
10.1046/j.1365-313X.1999.00588.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28864
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
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Scientific Publication
A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants
20
Arazi, T., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Sunkar, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Kaplan, B., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Fromm, H., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Centre for Plant Sciences, Leeds Inst. of Biotech. and Agric., University of Leeds, Leeds LS2 9JT, United Kingdom
A tobacco plasma membrane calmodulin-binding transporter confers Ni2+ tolerance and Pb2+ hypersensitivity in transgenic plants
All organisms require a minimal amount of metal ions to sustain their metabolism, growth and development. At the same time, their intrinsic metal-uptake systems render them vulnerable to toxic levels of metals in the biosphere. Using radiolabeled recombinant calmodulin as a probe to screen a tobacco cDNA library, we have discovered a protein designated NtCBP4 (Nicotiana tabacum calmodulin-binding protein) that can modulate plant tolerance to heavy metals. Structurally, NtCBP4 is similar to vertebrate and invertebrate K+ and to nonselective cation channels, as well as to recently reported proteins from barley and Arabidopsis. Here we report on the subcellular localization of NtCBP4 and the phenotype of transgenic plants overexpressing this protein. The localization of NtCBP4 in the plasma membrane was manifested by fractionating tobacco membranes on sucrose gradients or by aqueous two-phase partitioning, and subsequently using immunodetection. Several independent transgenic lines expressing NtCBP4 had higher than normal levels of NtCBP4. These transgenic lines were indistinguishable from wild type under normal growth conditions. However, they exhibited improved tolerance to Ni2+ and hypersensitivity to Pb2+, which are associated with reduced Ni2+ accumulation and enhanced Pb2+ accumulation, respectively. To our knowledge this is the first report of a plant protein that modulates plant tolerance or accumulation of Pb2+. We propose that NtCBP4 is involved in metal uptake across the plant plasma membrane. This gene may prove useful for implementing selective ion tolerance in crops and improving phytoremediation strategies.
Scientific Publication
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