Co-Authors:
Band, M., Agricultural Research Center, POB 6, Bet Dagan, 50250, Israel
Eggen, A., ABS Global Inc., 6908 River Road, Deforest, WI 53232, United States, Lab. Genet. Biochimique C., INRA CRJ, 78350 Jouyen-Josas, France
Bishop, M.D., ABS Global Inc., 6908 River Road, Deforest, WI 53232, United States
Ron, M., Agricultural Research Center, POB 6, Bet Dagan, 50250, Israel
Abstract:
A bovine yeast artificial chromosome (YAC) clone containing the superoxide dismutase 1 (SOD1) gene was used as a template for polymerase chain reaction (PCR) amplification using a conserved short interspersed nuclear element (SINE) primer. Two highly polymorphic microsatellites with nine and eight alleles were isolated and mapped by linkage analysis to the centromeric region of BTA1. These microsatellites will be used in the construction of a genetic and physical map of the SOD1 region towards positional cloning of the polled gene.
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