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Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche
Year:
1996
Source of publication :
Theoretical and Applied Genetics
Authors :
Joel, Daniel M.
;
.
Katzir, Nurit
;
.
Portnoy, Vitaly H.
;
.
Tzuri, Galil
;
.
Volume :
93
Co-Authors:
Katzir, N., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Portnoy, V., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Tzuri, G., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Castejón-Muñoz, M., Institute of Sustainable Agriculture, Apartado 4084, 14080 Córdoba, Spain
Joel, D.M., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Facilitators :
From page:
367
To page:
372
(
Total pages:
6
)
Abstract:
Despite the tremendous economic impact of broomrapes (Orobanche spp.) on agriculture in many countries little is known of the pattern of genetic variation within this group of parasitic weeds. The present paper describes the use of RAPD markers for the study of five Orobanche species in agricultural fields in Israel. Pronounced genetic differentiation was found between the species, and RAPD markers were raised for the identification of each of them. Southern-hybridization patterns of RAPD products of the various species were used to confirm the interpretation. The same markers were valid both for broomrapes collected in agricultural fields and for those collected in natural habitats. The validity of the markers found for O. cumana and O. crenata was confirmed on plants of the same species that were collected in Spain. Parsimony analysis of 86 RAPD characters produced a tree that clearly distinguishes between the five studied Orobanche species, separates the two Orobanche species belonging to sect. Trionychon from those belonging to sect. Osproleon, and supports the separation of O. cumana from O. cernua and of O. aegyptiaca from O. ramosa.
Note:
Related Files :
broomrape
DNA fingerprinting
Orobanche
Parasitic weed
RAPD markers
Show More
Related Content
More details
DOI :
10.1007/s001220050290
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28878
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:42
Scientific Publication
Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche
93
Katzir, N., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Portnoy, V., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Tzuri, G., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Castejón-Muñoz, M., Institute of Sustainable Agriculture, Apartado 4084, 14080 Córdoba, Spain
Joel, D.M., Newe-Ya'ar Research Center, Agricultural Research Organization, Haifa 31900, Israel
Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche
Despite the tremendous economic impact of broomrapes (Orobanche spp.) on agriculture in many countries little is known of the pattern of genetic variation within this group of parasitic weeds. The present paper describes the use of RAPD markers for the study of five Orobanche species in agricultural fields in Israel. Pronounced genetic differentiation was found between the species, and RAPD markers were raised for the identification of each of them. Southern-hybridization patterns of RAPD products of the various species were used to confirm the interpretation. The same markers were valid both for broomrapes collected in agricultural fields and for those collected in natural habitats. The validity of the markers found for O. cumana and O. crenata was confirmed on plants of the same species that were collected in Spain. Parsimony analysis of 86 RAPD characters produced a tree that clearly distinguishes between the five studied Orobanche species, separates the two Orobanche species belonging to sect. Trionychon from those belonging to sect. Osproleon, and supports the separation of O. cumana from O. cernua and of O. aegyptiaca from O. ramosa.
Scientific Publication
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