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Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco
Year:
2012
Authors :
Belausov, Eduard
;
.
Evenor, Dalia
;
.
Gaiduk, Svetlana
;
.
Keren, Ido
;
.
Ostersetzer, Oren
;
.
Reuveni, Moshe
;
.
Shaya, Felix
;
.
Shevtsov, Sofia
;
.
Zemach, Hanita
;
.
Volume :
54
Co-Authors:

Shaya, F., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Gaiduk, S., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Keren, I., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel, Department of Plant Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Shevtsov, S., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Zemah, H., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Evenor, D., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Reuveni, M., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Ostersetzer-Biran, O., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel, Australian Research Council (ARC), Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, WA 6009, Australia

Facilitators :
From page:
115
To page:
130
(
Total pages:
16
)
Abstract:
Plant mitochondrial genomes (mtDNAs) are large and undergo frequent recombination events. A common phenotype that emerges as a consequence of altered mtDNA structure is cytoplasmic-male sterility (CMS). The molecular basis for CMS remains unclear, but it seems logical that altered respiration activities would result in reduced pollen production. Analysis of tobacco (Nicotiana tabacum) mtDNAs indicated that CMS-associated loci often contain fragments of known organellar genes. These may assemble with organellar complexes and thereby interfere with normal respiratory functions. Here, we analyzed whether the expression of truncated fragments of mitochondrial genes (i.e. atp4, cox1 and rps3) may induce male sterility by limiting the biogenesis of the respiratory machinery. cDNA fragments corresponding to atp4f, cox1f and rps3f were cloned in-frame to a mitochondrial localization signal and a C-termini HA-tag under a tapetum-specific promoter and introduced to tobacco plants by Agrobacterium-mediated transformation. The constructs were then analyzed for their effect on mitochondrial activity and pollen fertility. Atp4f, Cox1f and Rps3f plants demonstrated male sterility phenotypes, which were tightly correlated with the expression of the recombinant fragments in the floral meristem. Fractionation of native organellar extracts showed that the recombinant ATP4f-HA, COX1f-HA and RPS3f-HA proteins are found in large membrane-associated particles. Analysis of the respiratory activities and protein profiles indicated that organellar complex I was altered in Atp4f, Cox1f and Rps3f plants. © 2012 Institute of Botany, Chinese Academy of Sciences.
Note:
Related Files :
Agrobacterium
DNA
gene expression
genetic recombination
Genetics
metabolism
phenotype
pollen (external)
Show More
Related Content
More details
DOI :
10.1111/j.1744-7909.2012.01099.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28940
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
You may also be interested in
Scientific Publication
Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco
54

Shaya, F., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Gaiduk, S., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Keren, I., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel, Department of Plant Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Shevtsov, S., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Zemah, H., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Evenor, D., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Reuveni, M., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel
Ostersetzer-Biran, O., Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel, Australian Research Council (ARC), Centre of Excellence in Plant Energy Biology, The University of Western Australia, Crawley, WA 6009, Australia

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco
Plant mitochondrial genomes (mtDNAs) are large and undergo frequent recombination events. A common phenotype that emerges as a consequence of altered mtDNA structure is cytoplasmic-male sterility (CMS). The molecular basis for CMS remains unclear, but it seems logical that altered respiration activities would result in reduced pollen production. Analysis of tobacco (Nicotiana tabacum) mtDNAs indicated that CMS-associated loci often contain fragments of known organellar genes. These may assemble with organellar complexes and thereby interfere with normal respiratory functions. Here, we analyzed whether the expression of truncated fragments of mitochondrial genes (i.e. atp4, cox1 and rps3) may induce male sterility by limiting the biogenesis of the respiratory machinery. cDNA fragments corresponding to atp4f, cox1f and rps3f were cloned in-frame to a mitochondrial localization signal and a C-termini HA-tag under a tapetum-specific promoter and introduced to tobacco plants by Agrobacterium-mediated transformation. The constructs were then analyzed for their effect on mitochondrial activity and pollen fertility. Atp4f, Cox1f and Rps3f plants demonstrated male sterility phenotypes, which were tightly correlated with the expression of the recombinant fragments in the floral meristem. Fractionation of native organellar extracts showed that the recombinant ATP4f-HA, COX1f-HA and RPS3f-HA proteins are found in large membrane-associated particles. Analysis of the respiratory activities and protein profiles indicated that organellar complex I was altered in Atp4f, Cox1f and Rps3f plants. © 2012 Institute of Botany, Chinese Academy of Sciences.
Scientific Publication
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