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De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel
Year:
2013
Source of publication :
BMC Genomics
Authors :
Aly, Radi
;
.
Droby, Samir
;
.
Kessler (Ben-Dayan), Clarita
;
.
Rafael, Ginat
;
.
Sela, Noa
;
.
Volume :
14
Co-Authors:
Hershkovitz, V., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Sela, N., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Taha-Salaime, L., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Pathology and Weed Research, The Volcani Center, Newe-Yaar Research Center, Ramat Yeshai 30095, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment,, The Hebrew University of Jerusalem, Revovot 76100, Israel
Liu, J., U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Rafael, G., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Kessler, C., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Aly, R., Department of Plant Pathology and Weed Research, The Volcani Center, Newe-Yaar Research Center, Ramat Yeshai 30095, Israel
Levy, M., Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment,, The Hebrew University of Jerusalem, Revovot 76100, Israel
Wisniewski, M., U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Droby, S., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background: The yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathogen, Penicillium digitatum.Results: More than 26 million sequencing reads were assembled into 9,674 unigenes. Approximately 50% of the unigenes could be annotated based on homology matches in the NCBI database. Based on homology, sequences were annotated with a gene description, gene ontology (GO term), and clustered into functional groups. An analysis of differential expression when the yeast was interacting with the fruit vs. the pathogen revealed more than 250 genes with specific expression responses. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. Patterns of gene expression in the two interactions were examined at the individual transcript level by quantitative real-time PCR analysis (RT-qPCR).Conclusion: This study provides new insight into the biology of the tritrophic interactions that occur in a biocontrol system such as the use of the yeast, M. fructicola for the control of green mold on citrus caused by P. digitatum. © 2013 Hershkovitz et al.; licensee BioMed Central Ltd.
Note:
Related Files :
Genetics
grapefruits
metabolism
molecular genetics
Penicillium
Show More
Related Content
More details
DOI :
10.1186/1471-2164-14-168
Article number:
168
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28957
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
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Scientific Publication
De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel
14
Hershkovitz, V., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Sela, N., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Taha-Salaime, L., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Pathology and Weed Research, The Volcani Center, Newe-Yaar Research Center, Ramat Yeshai 30095, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment,, The Hebrew University of Jerusalem, Revovot 76100, Israel
Liu, J., U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Rafael, G., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Kessler, C., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
Aly, R., Department of Plant Pathology and Weed Research, The Volcani Center, Newe-Yaar Research Center, Ramat Yeshai 30095, Israel
Levy, M., Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment,, The Hebrew University of Jerusalem, Revovot 76100, Israel
Wisniewski, M., U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Appalachian Fruit Research Station, Kearneysville, WV 25430, United States
Droby, S., Department of Postharvest and Food Sciences, ARO, The Volcani Center, Bet Dagan, 50250, Israel
De-novo assembly and characterization of the transcriptome of Metschnikowia fructicola reveals differences in gene expression following interaction with Penicillium digitatum and grapefruit peel
Background: The yeast Metschnikowia fructicola is an antagonist with biological control activity against postharvest diseases of several fruits. We performed a transcriptome analysis, using RNA-Seq technology, to examine the response of M. fructicola with citrus fruit and with the postharvest pathogen, Penicillium digitatum.Results: More than 26 million sequencing reads were assembled into 9,674 unigenes. Approximately 50% of the unigenes could be annotated based on homology matches in the NCBI database. Based on homology, sequences were annotated with a gene description, gene ontology (GO term), and clustered into functional groups. An analysis of differential expression when the yeast was interacting with the fruit vs. the pathogen revealed more than 250 genes with specific expression responses. In the antagonist-pathogen interaction, genes related to transmembrane, multidrug transport and to amino acid metabolism were induced. In the antagonist-fruit interaction, expression of genes involved in oxidative stress, iron homeostasis, zinc homeostasis, and lipid metabolism were induced. Patterns of gene expression in the two interactions were examined at the individual transcript level by quantitative real-time PCR analysis (RT-qPCR).Conclusion: This study provides new insight into the biology of the tritrophic interactions that occur in a biocontrol system such as the use of the yeast, M. fructicola for the control of green mold on citrus caused by P. digitatum. © 2013 Hershkovitz et al.; licensee BioMed Central Ltd.
Scientific Publication
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