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Combination of SCAR primers and Touchdown-PCR for sex identification in Pistacia vera L.
Year:
2005
Source of publication :
Scientia Horticulturae
Authors :
Barazani, Oz
;
.
Volume :
103
Co-Authors:
Yakubov, B., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Barazani, O., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Golan-Goldhirsh, A., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Facilitators :
From page:
473
To page:
478
(
Total pages:
6
)
Abstract:
A combined method of sequence characterized amplified regions (SCAR) primers and Touchdown-PCR was used for the development of a female DNA marker in Pistacia vera L. The random decamer primer OPO-08 amplified a 905-bp fragment in all female trees, but also in several males. SCAR primers designed on the basis of the RAPD female molecular marker amplified a 905-bp female and a 909-bp male fragment. Sequencing these fragments revealed high homology, with several point mutations, four deletions in female and one in the male sequences. A second internal set of SCAR primers designed on the basis of a polymorphic locus, in combination with Touchdown-PCR technique, amplified a specific female 297-bp product. The diagnostic reliability of the new female specific marker was verified on 54 different genotypes. The method reported here offers a simple and reproducible way for early gender determination in P. vera. © 2004 Elsevier B.V. All rights reserved.
Note:
Related Files :
Dioecy
Gender identification
Pistacia
Pistacia vera
Polymerase Chain Reaction
RAPD
Sex marker
Touchdown-PCR
Show More
Related Content
More details
DOI :
10.1016/j.scienta.2004.06.008
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28960
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
Scientific Publication
Combination of SCAR primers and Touchdown-PCR for sex identification in Pistacia vera L.
103
Yakubov, B., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Barazani, O., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Golan-Goldhirsh, A., Albert Katz Dept. Dryland B., Desert Plant Biotech. Laboratory, Jacob Blaustein Inst. Desert R., Israel
Combination of SCAR primers and Touchdown-PCR for sex identification in Pistacia vera L.
A combined method of sequence characterized amplified regions (SCAR) primers and Touchdown-PCR was used for the development of a female DNA marker in Pistacia vera L. The random decamer primer OPO-08 amplified a 905-bp fragment in all female trees, but also in several males. SCAR primers designed on the basis of the RAPD female molecular marker amplified a 905-bp female and a 909-bp male fragment. Sequencing these fragments revealed high homology, with several point mutations, four deletions in female and one in the male sequences. A second internal set of SCAR primers designed on the basis of a polymorphic locus, in combination with Touchdown-PCR technique, amplified a specific female 297-bp product. The diagnostic reliability of the new female specific marker was verified on 54 different genotypes. The method reported here offers a simple and reproducible way for early gender determination in P. vera. © 2004 Elsevier B.V. All rights reserved.
Scientific Publication
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