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Antigenic analysis of gamonts of Hepatozoon canis purified from leukocytes
Year:
2000
Source of publication :
Journal of Parasitology
Authors :
Samish, Michael
;
.
Volume :
86
Co-Authors:
Baneth, G., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shkap, V., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Jaffe, C.L., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Facilitators :
From page:
289
To page:
294
(
Total pages:
6
)
Abstract:
Hepatozoon canis is a tick-borne apicomplexan parasite of dogs that infects neutrophils and parenchymal tissues. To study the antigenic characteristics of this parasite, a technique was devised for the purification of gamonts from peripheral blood neutrophils. White blood cells were separated on Ficoll-Hypaque density gradients and the gamonts were released from the host neutrophils by nitrogen cavitation. The blood used for purification originated from dogs with natural or experimental infections of H. canis with a parasitemia of 1.4-33%. The number of parasites collected ranged from 1.5 x 106 to 4.2 x 107. Portions of purified gamonts were separated and examined under phase and scanning electron microscopy, and the remaining purified parasites were then used as a source of antigens to characterize the humoral immune response by western blot analysis. Serum antibodies from infected dogs recognized more than 15 gamont antigens, and the antigenic patterns observed with sera from naturally and experimentally infected dogs were nearly similar. Four immunodominant protein bands of relative molecular weights of 107, 88, 63, and 28 kDa were recognized by all of the sera examined. The technique applied here for the isolation of host cell-free gamonts will facilitate studies on antigenic composition and immune responses against H. canis and on antigenic relationships between Hepatozoon from different host species and geographic regions.
Note:
Related Files :
Acari
Animals
antigen detection
Antigens, Protozoan
Dog Diseases
Dogs
Eucoccidiida
Microscopy, Phase-Contrast
Neutrophils
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Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28989
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
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Scientific Publication
Antigenic analysis of gamonts of Hepatozoon canis purified from leukocytes
86
Baneth, G., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Shkap, V., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Jaffe, C.L., School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Department of Parasitology, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Antigenic analysis of gamonts of Hepatozoon canis purified from leukocytes
Hepatozoon canis is a tick-borne apicomplexan parasite of dogs that infects neutrophils and parenchymal tissues. To study the antigenic characteristics of this parasite, a technique was devised for the purification of gamonts from peripheral blood neutrophils. White blood cells were separated on Ficoll-Hypaque density gradients and the gamonts were released from the host neutrophils by nitrogen cavitation. The blood used for purification originated from dogs with natural or experimental infections of H. canis with a parasitemia of 1.4-33%. The number of parasites collected ranged from 1.5 x 106 to 4.2 x 107. Portions of purified gamonts were separated and examined under phase and scanning electron microscopy, and the remaining purified parasites were then used as a source of antigens to characterize the humoral immune response by western blot analysis. Serum antibodies from infected dogs recognized more than 15 gamont antigens, and the antigenic patterns observed with sera from naturally and experimentally infected dogs were nearly similar. Four immunodominant protein bands of relative molecular weights of 107, 88, 63, and 28 kDa were recognized by all of the sera examined. The technique applied here for the isolation of host cell-free gamonts will facilitate studies on antigenic composition and immune responses against H. canis and on antigenic relationships between Hepatozoon from different host species and geographic regions.
Scientific Publication
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