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The Complete Genomic Sequence of Pepper Yellow Leaf Curl Virus (PYLCV) and Its Implications for Our Understanding of Evolution Dynamics in the Genus Polerovirus
Year:
2013
Source of publication :
PLoS ONE
Authors :
Antignus, Yeheskel
;
.
Dombrovsky, Aviv
;
.
Doron-Faigenboim, Adi
;
.
Glanz, Eyal
;
.
Lachman, Oded
;
.
Sela, Noa
;
.
Volume :
8
Co-Authors:
Dombrovsky, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Glanz, E., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Lachman, O., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Sela, N., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Doron-Faigenboim, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Antignus, Y., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV). PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV) and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV) (both poleroviruses). The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs), which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93%) and to ORF5 of CABYV (87%). Both PYLCV and Pepper vein yellowing virus (PeVYV) contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP), may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2) were identified between nucleotides 4,962 and 5,061 (ORF 5) and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3) with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s) between poleroviruses co-infecting a common host(s), resulting in the emergence of PYLCV, a novel pathogen with a wider host range. © 2013 Dombrovsky et al.
Note:
Related Files :
gene sequence
genetic recombination
Molecular Evolution
Plant Diseases
sequence analysis
virus RNA
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Related Content
More details
DOI :
10.1371/journal.pone.0070722
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29022
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
You may also be interested in
Scientific Publication
The Complete Genomic Sequence of Pepper Yellow Leaf Curl Virus (PYLCV) and Its Implications for Our Understanding of Evolution Dynamics in the Genus Polerovirus
8
Dombrovsky, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Glanz, E., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Lachman, O., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Sela, N., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Doron-Faigenboim, A., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
Antignus, Y., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan, Israel
The Complete Genomic Sequence of Pepper Yellow Leaf Curl Virus (PYLCV) and Its Implications for Our Understanding of Evolution Dynamics in the Genus Polerovirus
We determined the complete sequence and organization of the genome of a putative member of the genus Polerovirus tentatively named Pepper yellow leaf curl virus (PYLCV). PYLCV has a wider host range than Tobacco vein-distorting virus (TVDV) and has a close serological relationship with Cucurbit aphid-borne yellows virus (CABYV) (both poleroviruses). The extracted viral RNA was subjected to SOLiD next-generation sequence analysis and used as a template for reverse transcription synthesis, which was followed by PCR amplification. The ssRNA genome of PYLCV includes 6,028 nucleotides encoding six open reading frames (ORFs), which is typical of the genus Polerovirus. Comparisons of the deduced amino acid sequences of the PYLCV ORFs 2-4 and ORF5, indicate that there are high levels of similarity between these sequences to ORFs 2-4 of TVDV (84-93%) and to ORF5 of CABYV (87%). Both PYLCV and Pepper vein yellowing virus (PeVYV) contain sequences that point to a common ancestral polerovirus. The recombination breakpoint which is located at CABYV ORF3, which encodes the viral coat protein (CP), may explain the CABYV-like sequences found in the genomes of the pepper infecting viruses PYLCV and PeVYV. Two additional regions unique to PYLCV (PY1 and PY2) were identified between nucleotides 4,962 and 5,061 (ORF 5) and between positions 5,866 and 6,028 in the 3' NCR. Sequence analysis of the pepper-infecting PeVYV revealed three unique regions (Pe1-Pe3) with no similarity to other members of the genus Polerovirus. Genomic analyses of PYLCV and PeVYV suggest that the speciation of these viruses occurred through putative recombination event(s) between poleroviruses co-infecting a common host(s), resulting in the emergence of PYLCV, a novel pathogen with a wider host range. © 2013 Dombrovsky et al.
Scientific Publication
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