נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Comparison of a novel tomato sucrose synthase, SlSUS4, with previously described SlSUS isoforms reveals distinct sequence features and differential expression patterns in association with stem maturation
Year:
2011
Source of publication :
Planta
Authors :
Goren, Shlomo
;
.
Granot, David
;
.
Volume :
233
Co-Authors:
Goren, S., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, Faculty of Agriculture, Food and Environment, Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, 76100 Rehovot, Israel
Huber, S.C., Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
1011
To page:
1023
(
Total pages:
13
)
Abstract:
Sucrose synthase (SUS) plays a role in many contexts of sugar metabolism, including low-oxygen and low-ATP respiration and the synthesis of cellulose. In tomato (Solanum lycopersicum), as in many plants, SUS is encoded by genes at several independent loci. Here, we report the isolation of a novel tomato SUS (SlSUS) isoform, SlSUS4, that is homologous to potato SUS isoform 1 (StSUS1) and also shows greater homology to SUS isoforms of other plants than to the other tomato SUS isoforms. All three tomato isoforms are very similar in genomic structure and sequence, yet each is located on a separate chromosome. Real-time expression analysis of the three distinct isoforms revealed widely varying patterns of expression, in terms of both tissue specificity and overall magnitude of expression. Analysis of SlSUS expression along the tomato stem revealed opposing expression gradients for two of the SlSUS isoforms, in apparent correlation with vascular tissue maturation. Western-blot analysis of SlSUS protein showed an increasing SlSUS concentration gradient along the developmental axis of the tomato stem, with the protein concentrated mainly in the vascular tissue of the stem. These gene expression and protein accumulation patterns indicate that each isoform may play a discrete role in the development of tomato plants, most notably in the development of vascular tissue in the stem. © 2011 Springer-Verlag.
Note:
Related Files :
biosynthesis
gene expression
Genetics
molecular genetics
Solanum
Solanum tuberosum
Show More
Related Content
More details
DOI :
10.1007/s00425-011-1356-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29031
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:43
You may also be interested in
Scientific Publication
Comparison of a novel tomato sucrose synthase, SlSUS4, with previously described SlSUS isoforms reveals distinct sequence features and differential expression patterns in association with stem maturation
233
Goren, S., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, Faculty of Agriculture, Food and Environment, Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, 76100 Rehovot, Israel
Huber, S.C., Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Comparison of a novel tomato sucrose synthase, SlSUS4, with previously described SlSUS isoforms reveals distinct sequence features and differential expression patterns in association with stem maturation
Sucrose synthase (SUS) plays a role in many contexts of sugar metabolism, including low-oxygen and low-ATP respiration and the synthesis of cellulose. In tomato (Solanum lycopersicum), as in many plants, SUS is encoded by genes at several independent loci. Here, we report the isolation of a novel tomato SUS (SlSUS) isoform, SlSUS4, that is homologous to potato SUS isoform 1 (StSUS1) and also shows greater homology to SUS isoforms of other plants than to the other tomato SUS isoforms. All three tomato isoforms are very similar in genomic structure and sequence, yet each is located on a separate chromosome. Real-time expression analysis of the three distinct isoforms revealed widely varying patterns of expression, in terms of both tissue specificity and overall magnitude of expression. Analysis of SlSUS expression along the tomato stem revealed opposing expression gradients for two of the SlSUS isoforms, in apparent correlation with vascular tissue maturation. Western-blot analysis of SlSUS protein showed an increasing SlSUS concentration gradient along the developmental axis of the tomato stem, with the protein concentrated mainly in the vascular tissue of the stem. These gene expression and protein accumulation patterns indicate that each isoform may play a discrete role in the development of tomato plants, most notably in the development of vascular tissue in the stem. © 2011 Springer-Verlag.
Scientific Publication
You may also be interested in