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The tomato MIXTA-like transcription factor coordinates fruit epidermis conical cell development and cuticular lipid biosynthesis and assembly
Year:
2015
Source of publication :
Plant physiology (source)
Authors :
Alkan, Noam
;
.
Volume :
169
Co-Authors:
Lashbrooke, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Research and Innovation Centre, Foundation Edmund Mach, San Michele all’Adige, Trento, Italy, Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, South Africa
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Lotan, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Alkan, N., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Tsimbalist, T., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Rechav, K., Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel
Fernandez-Moreno, J.-P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Plant Breeding and Biotechnology, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Widemann, E., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Grausem, B., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Pinot, F., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Granell, A., Department of Plant Breeding and Biotechnology, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Costa, F., Research and Innovation Centre, Foundation Edmund Mach, San Michele all’Adige, Trento, Italy
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
2553
To page:
2571
(
Total pages:
19
)
Abstract:
The epidermis of aerial plant organs is the primary source of building blocks forming the outer surface cuticular layer. To examine the relationship between epidermal cell development and cuticle assembly in the context of fruit surface, we investigated the tomato (Solanum lycopersicum) MIXTA-like gene. MIXTA/MIXTA-like proteins, initially described in snapdragon (Antirrhinum majus) petals, are known regulators of epidermal cell ifferentiation. Fruit of transgenically silenced SlMIXTA-like tomato plants displayed defects in patterning of conical epidermal cells. They also showed altered postharvest water loss and resistance to pathogens. Transcriptome and cuticular lipids profiling coupled with comprehensive microscopy revealed significant modifications to cuticle assembly and suggested SlMIXTA-like to regulate cutin biosynthesis. Candidate genes likely acting downstream of SlMIXTA-like included cytochrome P450s (CYPs) of the CYP77A and CYP86A subfamilies, LONG-CHAIN ACYL-COA SYNTHETASE2, GLYCEROL-3-PHOSPHATE SN-2-ACYLTRANSFERASE4, and the ATP-BINDING CASSETTE11 cuticular lipids transporter. As part of a larger regulatory network of epidermal cell patterning and L1-layer identity, we found that SlMIXTA-like acts downstream of SlSHINE3 and possibly cooperates with homeodomain Leu zipper IV transcription factors. Hence, SlMIXTA-like is a positive regulator of both cuticle and conical pidermal cell formation in tomato fruit, acting as a mediator of the tight association between fruit cutin polymer formation, uticle assembly, and epidermal cell patterning. ©2015 American Society of Plant Biologists. All rights reserved.
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DOI :
10.1104/pp.15.01145
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29062
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
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Scientific Publication
The tomato MIXTA-like transcription factor coordinates fruit epidermis conical cell development and cuticular lipid biosynthesis and assembly
169
Lashbrooke, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Research and Innovation Centre, Foundation Edmund Mach, San Michele all’Adige, Trento, Italy, Institute for Wine Biotechnology, Stellenbosch University, Stellenbosch, South Africa
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Lotan, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Alkan, N., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Tsimbalist, T., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
Rechav, K., Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel
Fernandez-Moreno, J.-P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel, Department of Plant Breeding and Biotechnology, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Widemann, E., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Grausem, B., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Pinot, F., Département Réseaux Métaboliques chez les Végétaux, Institut de Biologie Molééculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche, 2357, Université de Strasbourg, Strasbourg cedex, France
Granell, A., Department of Plant Breeding and Biotechnology, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Costa, F., Research and Innovation Centre, Foundation Edmund Mach, San Michele all’Adige, Trento, Italy
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel
The tomato MIXTA-like transcription factor coordinates fruit epidermis conical cell development and cuticular lipid biosynthesis and assembly
The epidermis of aerial plant organs is the primary source of building blocks forming the outer surface cuticular layer. To examine the relationship between epidermal cell development and cuticle assembly in the context of fruit surface, we investigated the tomato (Solanum lycopersicum) MIXTA-like gene. MIXTA/MIXTA-like proteins, initially described in snapdragon (Antirrhinum majus) petals, are known regulators of epidermal cell ifferentiation. Fruit of transgenically silenced SlMIXTA-like tomato plants displayed defects in patterning of conical epidermal cells. They also showed altered postharvest water loss and resistance to pathogens. Transcriptome and cuticular lipids profiling coupled with comprehensive microscopy revealed significant modifications to cuticle assembly and suggested SlMIXTA-like to regulate cutin biosynthesis. Candidate genes likely acting downstream of SlMIXTA-like included cytochrome P450s (CYPs) of the CYP77A and CYP86A subfamilies, LONG-CHAIN ACYL-COA SYNTHETASE2, GLYCEROL-3-PHOSPHATE SN-2-ACYLTRANSFERASE4, and the ATP-BINDING CASSETTE11 cuticular lipids transporter. As part of a larger regulatory network of epidermal cell patterning and L1-layer identity, we found that SlMIXTA-like acts downstream of SlSHINE3 and possibly cooperates with homeodomain Leu zipper IV transcription factors. Hence, SlMIXTA-like is a positive regulator of both cuticle and conical pidermal cell formation in tomato fruit, acting as a mediator of the tight association between fruit cutin polymer formation, uticle assembly, and epidermal cell patterning. ©2015 American Society of Plant Biologists. All rights reserved.
Scientific Publication
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