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Natural infection of banana by a satellite-containing strain of cucumber mosaic virus: Nucleotide sequence of the coat protein gene and the satellite RNA
Year:
1996
Source of publication :
Phytoparasitica
Authors :
Bar-Joseph, Moshe
;
.
Gafny, Ron
;
.
Mawassi, Munir
;
.
Weksler, Asya
;
.
Volume :
24
Co-Authors:
Gafny, R., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Wexler, A., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Israeli, Y., Jordan Valley Banana Res. Station, Zemach 15132, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
49
To page:
56
(
Total pages:
8
)
Abstract:
Banana plants expressing mosaic symptoms, from the Jordan Valley in Israel, were shown to be infected by a satellite-RNA-containing strain of cucumber mosaic virus (CMV). Double-stranded RNA isolated from from field-infected banana, without through host, was used as a template for synthesis of cDNA. The cDNAs corresponding to the coat protein (CP) gene and to the satellite RNA were cloned after polymerase chain reaction amplification. The nucleotide sequences of the CP and the satellite cDNAs were determined. The CP gene and its 3′ flanking sequence had 98% similarity to the CMV Fny nucleotide sequence and the two strains differed in only one amino acid of the CP. The associated satellite had a sequence similarity ranging from 95% to 85.6% with other CMV satellites. Analysis of banana suckers differing in symptoms' severity indicated a correlation between the presence of satellite and attenuation of symptoms.
Note:
Related Files :
banana
Cucumber mosaic virus
double-stranded RNA
Musa spp.
Polymerase chain reaction, PCR
satellite RNA
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29073
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
Scientific Publication
Natural infection of banana by a satellite-containing strain of cucumber mosaic virus: Nucleotide sequence of the coat protein gene and the satellite RNA
24
Gafny, R., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Wexler, A., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Israeli, Y., Jordan Valley Banana Res. Station, Zemach 15132, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Dept. of Virology, Volcani Center, Bet Dagan 50250, Israel
Natural infection of banana by a satellite-containing strain of cucumber mosaic virus: Nucleotide sequence of the coat protein gene and the satellite RNA
Banana plants expressing mosaic symptoms, from the Jordan Valley in Israel, were shown to be infected by a satellite-RNA-containing strain of cucumber mosaic virus (CMV). Double-stranded RNA isolated from from field-infected banana, without through host, was used as a template for synthesis of cDNA. The cDNAs corresponding to the coat protein (CP) gene and to the satellite RNA were cloned after polymerase chain reaction amplification. The nucleotide sequences of the CP and the satellite cDNAs were determined. The CP gene and its 3′ flanking sequence had 98% similarity to the CMV Fny nucleotide sequence and the two strains differed in only one amino acid of the CP. The associated satellite had a sequence similarity ranging from 95% to 85.6% with other CMV satellites. Analysis of banana suckers differing in symptoms' severity indicated a correlation between the presence of satellite and attenuation of symptoms.
Scientific Publication
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