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Identifying molecular genetic markers associated with seedlessness in grape
Year:
1996
Authors :
Ben Hayyim, Gosal
;
.
Spiegel-Roy, Pinchas
;
.
Volume :
121
Co-Authors:
Striem, M.J., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Ben-Hayyim, G., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Spiegel-Roy, P., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Facilitators :
From page:
758
To page:
763
(
Total pages:
6
)
Abstract:
Excluding seeded offspring at an early stage could be of great value to the breeder concerned with the development of seedless grapes (Vitis vinifera L.). We used the random amplified polymorphic DNA (RAPD) technique to identify molecular genetic markers, analyzing 82 individuals of a progeny resulting from a cross between 'Early Muscat' (seeded) and 'Flame Seedless'. Seven variables representing the traits of seedlessness were analyzed: mean fresh weight of one seed. Total fresh weight of seeds per berry, perception of seed content, seed size categories evaluated visually, degree of hardness of the seed coat, degree of development of the endosperm, and degree of development of the embryo. Among 160 10mer primers, 110 gave distinct band patterns. Twelve markers yielded significant correlations with several subtraits of seedlessness, mainly with the mean fresh weight of one seed and the total fresh weight of seeds per berry. Multiple linear regression analysis resulted in high coefficients, such as R = 0.779 for fresh weight of seeds per berry, when the seven markers were included as independent variables in the model. Most of the seeded individuals, about 44% of the progeny, could be excluded using a two-step process of marker assisted selection.
Note:
Related Files :
breeding
PCR
preselection
random amplified polymorphic DNA
stenospermocarpy
Vitaceae
Vitis sp.
Vitis vinifera
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29078
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
Scientific Publication
Identifying molecular genetic markers associated with seedlessness in grape
121
Striem, M.J., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Ben-Hayyim, G., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Spiegel-Roy, P., Dept. Fruit Tree Breed. and Genet., Institute of Horticulture, Volcani Center, Bet Dagan 50-250, Israel
Identifying molecular genetic markers associated with seedlessness in grape
Excluding seeded offspring at an early stage could be of great value to the breeder concerned with the development of seedless grapes (Vitis vinifera L.). We used the random amplified polymorphic DNA (RAPD) technique to identify molecular genetic markers, analyzing 82 individuals of a progeny resulting from a cross between 'Early Muscat' (seeded) and 'Flame Seedless'. Seven variables representing the traits of seedlessness were analyzed: mean fresh weight of one seed. Total fresh weight of seeds per berry, perception of seed content, seed size categories evaluated visually, degree of hardness of the seed coat, degree of development of the endosperm, and degree of development of the embryo. Among 160 10mer primers, 110 gave distinct band patterns. Twelve markers yielded significant correlations with several subtraits of seedlessness, mainly with the mean fresh weight of one seed and the total fresh weight of seeds per berry. Multiple linear regression analysis resulted in high coefficients, such as R = 0.779 for fresh weight of seeds per berry, when the seven markers were included as independent variables in the model. Most of the seeded individuals, about 44% of the progeny, could be excluded using a two-step process of marker assisted selection.
Scientific Publication
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