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Differentiating PVY(NTN) by unique single-restriction cleavage of PCR products
Year:
1999
Source of publication :
Potato Research
Authors :
Maslenin, Ludmila
;
.
Rosner, Arie
;
.
Volume :
42
Co-Authors:
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
215
To page:
221
(
Total pages:
7
)
Abstract:
A procedure for differentiating PVY(NTN) from PVY(N) is described and is based on the unique cleavage of their respective PCR products with strain specific restriction endonucleases. The PCR products corresponding to the 5' end of the N and NTN strains of PVY were cloned and sequenced, and a restriction map was constructed which included common enzymes that were used for the differentiation of PVY(NTN.) Unique, single cleavage of PCR products derived from the 5' end of the PVY(NTN) genome by Nco I, and that of the N-strain of PVY by Bgl II restriction endonuclease were demonstrated. The specific digestion patterns in polyacrylamide gel were used for the unequivocal differentiation between the N and NTN strains of the virus. Both single and mixed infections were detected in field samples of potatoes using this procedure.
Note:
Related Files :
restriction endonuclease
Solanum tuberosum
Strain differentiation
virus genome
virus infection
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29147
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
Scientific Publication
Differentiating PVY(NTN) by unique single-restriction cleavage of PCR products
42
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Differentiating PVY(NTN) by unique single-restriction cleavage of PCR products
A procedure for differentiating PVY(NTN) from PVY(N) is described and is based on the unique cleavage of their respective PCR products with strain specific restriction endonucleases. The PCR products corresponding to the 5' end of the N and NTN strains of PVY were cloned and sequenced, and a restriction map was constructed which included common enzymes that were used for the differentiation of PVY(NTN.) Unique, single cleavage of PCR products derived from the 5' end of the PVY(NTN) genome by Nco I, and that of the N-strain of PVY by Bgl II restriction endonuclease were demonstrated. The specific digestion patterns in polyacrylamide gel were used for the unequivocal differentiation between the N and NTN strains of the virus. Both single and mixed infections were detected in field samples of potatoes using this procedure.
Scientific Publication
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