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Identification and Molecular Characterization of Homeologous Δ9-Stearoyl Acyl Carrier Protein Desaturase 3 Genes from the Allotetraploid Peanut (Arachis hypogaea)
Year:
2011
Source of publication :
Plant Molecular Biology Reporter
Authors :
Brand, Arnon
;
.
Brand, Yael
;
.
Hovav, Ran H.
;
.
Shilman, Florin
;
.
Volume :
29
Co-Authors:
Shilman, F., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Brand, Y., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Brand, A., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Hedvat, I., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Hovav, R., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Facilitators :
From page:
232
To page:
241
(
Total pages:
10
)
Abstract:
The stearoyl-acyl carrier protein (ACP) desaturase (SAD) is a nuclear-encoded, plastid-localized soluble desaturase that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP and plays a key role in the determination of the properties of the majority of cellular glycerolipids. Sad genes from a variety of plant species have been cloned and characterized. However, in peanut (Arachis hypogaea), an important edible and oilseed crop, these genes have not yet been characterized. By searching peanut expressed sequence tag (EST) and parallel sequencing (454) libraries, we have identified three members of the ahSad gene family. Among them, only one gene, ahSad3, was exclusively expressed during seed development and in a manner fully corresponding to oil accumulation. Both ahSad3 homeologous genes (ahSad3A and ahSad3B) were recovered from the allotetraploid peanut, and their mRNA expression levels were characterized. The open reading frames for ahSad3A and ahSad3B are 98% identical and consist of 1,158 bp, encoding a 386-full-amino-acid protein, with one intron in the coding sequence. Comparisons of the sequences of these two homeologous genes revealed seven single-nucleotide polymorphisms and one triplet insertion in the coding region. Southern blot analysis indicated that there are only two copies of the ahSad3 gene in the peanut genome. Homeolog-specific gene expression analysis showed that both ahSad3 homeologs are expressed in developing seeds, but gene expression is significantly biased toward the B genome. Our results point to ahSad3 as a possible target gene for manipulation of fatty acid saturation in A. hypogaea. © 2010 Springer-Verlag.
Note:
Related Files :
Arachis hypogaea
Homeologous genes
Real-time PCR
Southern blot
Stearoyl-acyl carrier protein desaturase
Show More
Related Content
More details
DOI :
10.1007/s11105-010-0226-9
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29173
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:44
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Scientific Publication
Identification and Molecular Characterization of Homeologous Δ9-Stearoyl Acyl Carrier Protein Desaturase 3 Genes from the Allotetraploid Peanut (Arachis hypogaea)
29
Shilman, F., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Brand, Y., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Brand, A., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Hedvat, I., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Hovav, R., Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Identification and Molecular Characterization of Homeologous Δ9-Stearoyl Acyl Carrier Protein Desaturase 3 Genes from the Allotetraploid Peanut (Arachis hypogaea)
The stearoyl-acyl carrier protein (ACP) desaturase (SAD) is a nuclear-encoded, plastid-localized soluble desaturase that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP and plays a key role in the determination of the properties of the majority of cellular glycerolipids. Sad genes from a variety of plant species have been cloned and characterized. However, in peanut (Arachis hypogaea), an important edible and oilseed crop, these genes have not yet been characterized. By searching peanut expressed sequence tag (EST) and parallel sequencing (454) libraries, we have identified three members of the ahSad gene family. Among them, only one gene, ahSad3, was exclusively expressed during seed development and in a manner fully corresponding to oil accumulation. Both ahSad3 homeologous genes (ahSad3A and ahSad3B) were recovered from the allotetraploid peanut, and their mRNA expression levels were characterized. The open reading frames for ahSad3A and ahSad3B are 98% identical and consist of 1,158 bp, encoding a 386-full-amino-acid protein, with one intron in the coding sequence. Comparisons of the sequences of these two homeologous genes revealed seven single-nucleotide polymorphisms and one triplet insertion in the coding region. Southern blot analysis indicated that there are only two copies of the ahSad3 gene in the peanut genome. Homeolog-specific gene expression analysis showed that both ahSad3 homeologs are expressed in developing seeds, but gene expression is significantly biased toward the B genome. Our results point to ahSad3 as a possible target gene for manipulation of fatty acid saturation in A. hypogaea. © 2010 Springer-Verlag.
Scientific Publication
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