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Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel
Year:
2010
Source of publication :
Phytoparasitica
Authors :
Glazer, Itamar
;
.
Salame, Liora
;
.
Volume :
38
Co-Authors:
Salame, L., Nematology Unit, Department of Entomology, ARO, The Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Nematology Unit, Department of Entomology, ARO, The Volcani Center, Bet Dagan 50250, Israel
Miqaia, N., Department of Biological Control, Institute of Plant Protection, 82, Chavchavadze Ave, 0162 Tbilisi, Georgia
Chkhubianishvili, T., Department of Biological Control, Institute of Plant Protection, 82, Chavchavadze Ave, 0162 Tbilisi, Georgia
Facilitators :
From page:
39
To page:
52
(
Total pages:
14
)
Abstract:
Entomopathogenic nematodes (EPNs) are known as effective biological agents against soil-dwelling stages of insect pests. In the present study new EPN populations from the Steinernematidae and Heterorhabditidae families were isolated at different locations in Israel. The newly isolated populations were subjected to a series of bioassays for beneficial traits, for selection of a superior isolate for biological control of insect pests. Nematode motility was measured in sand columns, with and without the susceptible insect Galleria mellonella. Infectivity was evaluated using an invasion rate assay, as well as a dose response assay in which LD50 values were calculated. Nematode tolerance to environmental stresses was examined in heat and desiccation tolerance assays. The reproductive potentials of the new isolates were assessed in cadavers of G. mellonella. All Steinernematid populations were identified as Steinernema feltiae and the Heterorhabditids as Heterorhabditis sp. Large variation between the various populations was recorded in all assays. In order to identify a superior isolate, each strain was scored as 1 if its performance did not differ significantly from the highest level recorded in the particular assay; -1 if its performance did not differ significantly from the lowest level; and 0 if its performance was between the highest and lowest levels or did not differ from either. The scores for the different traits were then summed for each population and the totals were compared. The best score (+4) was obtained for a S. feltiae population isolated in northern Israel. These results will be used for the utilization of the new population as a biological control agent. © Springer Science & Business Media B.V. 2009.
Note:
Related Files :
biological control
heat tolerance
Heterorhabditidae
motility
Nematoda
Reproduction
Sand columns
Steinernema feltiae
Show More
Related Content
More details
DOI :
10.1007/s12600-009-0072-1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29212
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
You may also be interested in
Scientific Publication
Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel
38
Salame, L., Nematology Unit, Department of Entomology, ARO, The Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Nematology Unit, Department of Entomology, ARO, The Volcani Center, Bet Dagan 50250, Israel
Miqaia, N., Department of Biological Control, Institute of Plant Protection, 82, Chavchavadze Ave, 0162 Tbilisi, Georgia
Chkhubianishvili, T., Department of Biological Control, Institute of Plant Protection, 82, Chavchavadze Ave, 0162 Tbilisi, Georgia
Characterization of populations of entomopathogenic nematodes isolated at diverse sites across Israel
Entomopathogenic nematodes (EPNs) are known as effective biological agents against soil-dwelling stages of insect pests. In the present study new EPN populations from the Steinernematidae and Heterorhabditidae families were isolated at different locations in Israel. The newly isolated populations were subjected to a series of bioassays for beneficial traits, for selection of a superior isolate for biological control of insect pests. Nematode motility was measured in sand columns, with and without the susceptible insect Galleria mellonella. Infectivity was evaluated using an invasion rate assay, as well as a dose response assay in which LD50 values were calculated. Nematode tolerance to environmental stresses was examined in heat and desiccation tolerance assays. The reproductive potentials of the new isolates were assessed in cadavers of G. mellonella. All Steinernematid populations were identified as Steinernema feltiae and the Heterorhabditids as Heterorhabditis sp. Large variation between the various populations was recorded in all assays. In order to identify a superior isolate, each strain was scored as 1 if its performance did not differ significantly from the highest level recorded in the particular assay; -1 if its performance did not differ significantly from the lowest level; and 0 if its performance was between the highest and lowest levels or did not differ from either. The scores for the different traits were then summed for each population and the totals were compared. The best score (+4) was obtained for a S. feltiae population isolated in northern Israel. These results will be used for the utilization of the new population as a biological control agent. © Springer Science & Business Media B.V. 2009.
Scientific Publication
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