נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a new set of microsatellite markers
Year:
2001
Source of publication :
Molecular Genetics and Genomics
Authors :
Lavi, Uri
;
.
Volume :
266
Co-Authors:
David, L., Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Sciences, Hebrew University of Jerusalem, P.O.Box 12, Rehovot 76100, Israel
Rajasekaran, P., Unit of Applied Genetics, Scottish Crop Research Institute, Invergowrie DD2 5DA Dundee, United Kingdom
Fang, J., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Hillel, J., Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Sciences, Hebrew University of Jerusalem, P.O.Box 12, Rehovot 76100, Israel
Lavi, U., Institute of Horticulture, ARO-Volcani Center, P.O.Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
353
To page:
362
(
Total pages:
10
)
Abstract:
Forty-seven new microsatellite markers were generated and applied, together with the AFLP (Amplified Fragment Length Polymorphism) technique using two different enzyme combinations, to the genetic analysis of two carp species, Cyprinus carpio L. and Ctenopharyngodon idella. The extent of polymorphism and the genetic relationships between nine carp populations were studied. The incidence of microsatellites containing CA and CT motifs was estimated to be one every 17.4 and one every 126.3 kb, respectively, and their average allele numbers were four and rive, respectively. Across populations, the average proportion of individuals that were heterozygous for microsatellite markers was 44.2% and the average allele number was 4.02. The EcoRI/TaqI combination generated more analyzable AFLP bands than the EcoRI/MseI pair, making the former preferable for the analysis of carp populations. The proportion of polymorphic AFLP bands within populations ranged from 6.7% in grass carp to 59.9% in Kohaku strain (Koi) of the ornamental carp. The fixation index (FST) for microsatellites in these populations was estimated to be 0.37, and for AFLP markers the value was 0.39. Genetic distance matrices derived from microsatellites and from two AFLP analyses were positively correlated. Grass carp showed fewer AFLP bands than other populations and was genotyped by only hall of the microsatellite markers. These findings agree with genetic distance estimates in suggesting that the grass carp is phylogenetically quite remote from all the other populations examined.
Note:
Related Files :
AFLP
Animals
chromosome mapping
Coprosma kauensis
Cyprinus carpio
DNA
genetic markers
Grass carp
Show More
Related Content
More details
DOI :
10.1007/s004380100569
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29223
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
You may also be interested in
Scientific Publication
Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a new set of microsatellite markers
266
David, L., Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Sciences, Hebrew University of Jerusalem, P.O.Box 12, Rehovot 76100, Israel
Rajasekaran, P., Unit of Applied Genetics, Scottish Crop Research Institute, Invergowrie DD2 5DA Dundee, United Kingdom
Fang, J., College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Hillel, J., Department of Field Crops and Genetics, Faculty of Agriculture, Food and Environmental Sciences, Hebrew University of Jerusalem, P.O.Box 12, Rehovot 76100, Israel
Lavi, U., Institute of Horticulture, ARO-Volcani Center, P.O.Box 6, Bet-Dagan 50250, Israel
Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a new set of microsatellite markers
Forty-seven new microsatellite markers were generated and applied, together with the AFLP (Amplified Fragment Length Polymorphism) technique using two different enzyme combinations, to the genetic analysis of two carp species, Cyprinus carpio L. and Ctenopharyngodon idella. The extent of polymorphism and the genetic relationships between nine carp populations were studied. The incidence of microsatellites containing CA and CT motifs was estimated to be one every 17.4 and one every 126.3 kb, respectively, and their average allele numbers were four and rive, respectively. Across populations, the average proportion of individuals that were heterozygous for microsatellite markers was 44.2% and the average allele number was 4.02. The EcoRI/TaqI combination generated more analyzable AFLP bands than the EcoRI/MseI pair, making the former preferable for the analysis of carp populations. The proportion of polymorphic AFLP bands within populations ranged from 6.7% in grass carp to 59.9% in Kohaku strain (Koi) of the ornamental carp. The fixation index (FST) for microsatellites in these populations was estimated to be 0.37, and for AFLP markers the value was 0.39. Genetic distance matrices derived from microsatellites and from two AFLP analyses were positively correlated. Grass carp showed fewer AFLP bands than other populations and was genotyped by only hall of the microsatellite markers. These findings agree with genetic distance estimates in suggesting that the grass carp is phylogenetically quite remote from all the other populations examined.
Scientific Publication
You may also be interested in