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Development of sensitive methods for the detection of myobacteria by DNA probes
Year:
1990
Source of publication :
FEMS Microbiology Letters
Authors :
Sela, Shlomo
;
.
Volume :
72
Co-Authors:
Rahav, G., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Sela, S., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Facilitators :
From page:
29
To page:
33
(
Total pages:
5
)
Abstract:
A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes. © 1990.
Note:
Related Files :
bacterial RNA
DNA, Ribosomal
dna dna hybridization
genetic engineering
genetic procedures
Genetics
RNA, Ribosomal
Show More
Related Content
More details
DOI :
10.1016/0378-1097(90)90339-R
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29235
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
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Development of sensitive methods for the detection of myobacteria by DNA probes
72
Rahav, G., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Sela, S., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Bercovier, H., Department of Clinical Microbiology, The Hebrew University - Hadassah Medical School, Jerusalem, Israel
Development of sensitive methods for the detection of myobacteria by DNA probes
A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes. © 1990.
Scientific Publication
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