נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Developmental toxicology of solamargine and solasonine glycoalkaloids in frog embryos
Year:
1998
Source of publication :
Food and Chemical Toxicology
Authors :
Weissenberg, Martin
;
.
Volume :
36
Co-Authors:
Blankemeyer, J.T., Department of Microbiology, Oklahoma State University, Stillwater, OK 74078, United States
McWilliams, M.L., Department of Microbiology, Oklahoma State University, Stillwater, OK 74078, United States
Rayburn, J.R., National Research Council Associate, c/o GED, US EPA, Gulf Breeze, FL 32561, United States
Weissenberg, M., Volcani Center, Agricultural Organization, Bet Dagan, Israel
Friedman, M., USDA-ARS W. Regional Research Center, 800 Buchanan Street, Albany, CA 94710, United States
Facilitators :
From page:
383
To page:
389
(
Total pages:
7
)
Abstract:
As part of an effort to improve the safety of plant foods, a need exists to define the relative toxicities of structurally different glycoalkaloids and metabolites which may be present in Solanum plant species such as potatoes, tomatoes and eggplants. The objectives of this study were to determine the relative toxicities and the modes of action of the eggplant (Solanum melongena) glycoalkaloids solamargine and solasonine in Xenopus laevis frog embryos, using membrane potential and embryo growth and teratogenicity assays. In the cell membrane assays, adverse effects on embryos were evaluated by measuring membrane potentials using an electrochromic dye, di-4-ANEPPS, as a fluorescence probe for the integrity of the membranes. In the embryo growth and teratogenesis assays, the survival of the embryos and organ malformations was used as an index of embryo toxicity. The relative potencies of glycoalkaloids are similar for frog embryo effects (survival and teratogenicities) and for membrane effects (membrane potential). Experiments with solasonine at pH 6 and 8 suggest that the unprotonated form of the glycoalkaloids appears to be involved in the membrane effects. The nature of the carbohydrate side-chains of the steroidal glycosides governs relative potencies. The possible significance of the findings to food safety and plant physiology and possible application of the membrane assays to bacterial toxins are discussed.
Note:
Related Files :
animal experiment
animal model
Animals
cell membrane
Phytosterols
Solanaceae
unclassified drug
Show More
Related Content
More details
DOI :
10.1016/S0278-6915(97)00164-6
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29276
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
You may also be interested in
Scientific Publication
Developmental toxicology of solamargine and solasonine glycoalkaloids in frog embryos
36
Blankemeyer, J.T., Department of Microbiology, Oklahoma State University, Stillwater, OK 74078, United States
McWilliams, M.L., Department of Microbiology, Oklahoma State University, Stillwater, OK 74078, United States
Rayburn, J.R., National Research Council Associate, c/o GED, US EPA, Gulf Breeze, FL 32561, United States
Weissenberg, M., Volcani Center, Agricultural Organization, Bet Dagan, Israel
Friedman, M., USDA-ARS W. Regional Research Center, 800 Buchanan Street, Albany, CA 94710, United States
Developmental toxicology of solamargine and solasonine glycoalkaloids in frog embryos
As part of an effort to improve the safety of plant foods, a need exists to define the relative toxicities of structurally different glycoalkaloids and metabolites which may be present in Solanum plant species such as potatoes, tomatoes and eggplants. The objectives of this study were to determine the relative toxicities and the modes of action of the eggplant (Solanum melongena) glycoalkaloids solamargine and solasonine in Xenopus laevis frog embryos, using membrane potential and embryo growth and teratogenicity assays. In the cell membrane assays, adverse effects on embryos were evaluated by measuring membrane potentials using an electrochromic dye, di-4-ANEPPS, as a fluorescence probe for the integrity of the membranes. In the embryo growth and teratogenesis assays, the survival of the embryos and organ malformations was used as an index of embryo toxicity. The relative potencies of glycoalkaloids are similar for frog embryo effects (survival and teratogenicities) and for membrane effects (membrane potential). Experiments with solasonine at pH 6 and 8 suggest that the unprotonated form of the glycoalkaloids appears to be involved in the membrane effects. The nature of the carbohydrate side-chains of the steroidal glycosides governs relative potencies. The possible significance of the findings to food safety and plant physiology and possible application of the membrane assays to bacterial toxins are discussed.
Scientific Publication
You may also be interested in