Co-Authors:
Guenoune, D., Agronomy and Natural Resources Department, Agricultural Research Organization, Volcani Center, POB, Bet Dagan 50250, Israel
Amir, R., Department of Plant Physiology, Migal Technological Center, Kiryat Shmona 12100, Israel
Badani, H., Agronomy and Natural Resources Department, Agricultural Research Organization, Volcani Center, POB, Bet Dagan 50250, Israel
Wolf, S., Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Galili, S., Agronomy and Natural Resources Department, Agricultural Research Organization, Volcani Center, POB, Bet Dagan 50250, Israel
Abstract:
Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.