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Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022
Year:
2005
Source of publication :
Plant Molecular Biology
Authors :
Maslenin, Ludmila
;
.
Rosner, Arie
;
.
Volume :
57
Co-Authors:
Gottfried, P., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Lotan, O., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Kolot, M., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel
Bendov, R., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Gorovits, R., Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Yesodi, V., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel
Facilitators :
From page:
435
To page:
444
(
Total pages:
10
)
Abstract:
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization. © Springer 2005.
Note:
Related Files :
arabidopsis
bacteria
cloning
DNA
genetic recombination
Genetics
Nucleic acids
Plants
Show More
Related Content
More details
DOI :
10.1007/s11103-004-0076-7
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29301
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:45
Scientific Publication
Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022
57
Gottfried, P., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Lotan, O., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Kolot, M., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel
Bendov, R., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Gorovits, R., Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Yesodi, V., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel
Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization. © Springer 2005.
Scientific Publication
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