אסיף מאגר המחקר החקלאי

Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

Year:

2005

Source of publication :

Authors :

Volume :

57

Co-Authors:

Gottfried, P., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Lotan, O., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Kolot, M., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Maslenin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel
Bendov, R., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Gorovits, R., Dept. Plant Pathol. and Microbiol., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Yesodi, V., FertiSeeds Ltd., 13A Building, K. Weizmann Science Park, Ness Ziona 70400, Israel
Yagil, E., Department of Biochemistry, Tel-Aviv University, Tel-Aviv 69978, Israel
Rosner, A., Department of Virology, Agricultural Research Organization, Volcani Center, Beit Dagan 50250, Israel

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Abstract:

The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP × attB) as well as a site-specific excision reaction (attL × attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization. © Springer 2005.

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