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Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: Effect of genotype and exogenous application of glycinebetaine
Year:
2009
Source of publication :
Journal of Experimental Botany
Authors :
Gollop, Natan
;
.
Heuer, Bruria
;
.
Volume :
60
Co-Authors:
Chen, S., Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Heuer, B., Department of Soil, Water and Environmental Sciences, ARO, Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
2005
To page:
2019
(
Total pages:
15
)
Abstract:
An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and 'F144', using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline control and salt-stressed seedlings, and separated by two-dimensional gel electrophoresis. Liqud chromatography-electron spray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified 23 salt stress response proteins, classified into six functional categories. The effect of exogenously applied glycinebetaine (GB) on the salt stress-induced inhibition of growth in tomato seedlings of cultivars Patio and 'F144' and on the protein profile was investigated. It was found that GB could alleviate the inhibition of tomato growth induced by salt stress through changing the expression abundance of six proteins in Patio and two proteins in 'F144' more than twice compared with salt-stressed seedlings. Furthermore, the interaction analysis based on computational bioinformatics reveals major regulating networks: photosystem II (PSII), Rubisco, and superoxide dismutase (SOD). The results suggest that it is likely that improvement of salt tolerance in tomato might be achieved through the application of exogenous compatible solutes, such as GB. Moreover, quantitative and qualitative analysis of the differentially expressed proteins of tomato under salt stress is an important step towards further elucidation of mechanisms of salt stress resistance.
Note:
Related Files :
bioinformatics
Exogenous application
Genetics
metabolism
proteomics
Solanum
Show More
Related Content
More details
DOI :
10.1093/jxb/erp075
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29340
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:46
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Scientific Publication
Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: Effect of genotype and exogenous application of glycinebetaine
60
Chen, S., Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Heuer, B., Department of Soil, Water and Environmental Sciences, ARO, Volcani Center, Bet-Dagan, Israel
Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: Effect of genotype and exogenous application of glycinebetaine
An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and 'F144', using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline control and salt-stressed seedlings, and separated by two-dimensional gel electrophoresis. Liqud chromatography-electron spray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified 23 salt stress response proteins, classified into six functional categories. The effect of exogenously applied glycinebetaine (GB) on the salt stress-induced inhibition of growth in tomato seedlings of cultivars Patio and 'F144' and on the protein profile was investigated. It was found that GB could alleviate the inhibition of tomato growth induced by salt stress through changing the expression abundance of six proteins in Patio and two proteins in 'F144' more than twice compared with salt-stressed seedlings. Furthermore, the interaction analysis based on computational bioinformatics reveals major regulating networks: photosystem II (PSII), Rubisco, and superoxide dismutase (SOD). The results suggest that it is likely that improvement of salt tolerance in tomato might be achieved through the application of exogenous compatible solutes, such as GB. Moreover, quantitative and qualitative analysis of the differentially expressed proteins of tomato under salt stress is an important step towards further elucidation of mechanisms of salt stress resistance.
Scientific Publication
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