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Differential expression of cucumber RNA-dependent RNA polymerase 1 genes during antiviral defence and resistance
Year:
2017
Source of publication :
Molecular Plant Pathology
Authors :
Haviv, Sabrina
;
.
Kravchik, Michael
;
.
Weissberg, Mira
;
.
Volume :
19
Co-Authors:
Leibman, D., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Kravchik, M., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Wolf, D., Department of Vegetable and Field CropsAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Haviv, S., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Weissberg, M., Department of Fruit Tree SciencesAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Ophir, R., Department of Fruit Tree SciencesAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Paris, H.S., Department of Vegetable Crops and Plant GeneticsAgricultural Research Organization, Newe Ya'ar Research CenterPO Box 1021Ramat 30-095Yishay Israel
Palukaitis, P., Department of Horticultural SciencesSeoul Women's UniversityNowon-gu 01797Seoul South Korea
Ding, S.-W., Department of Plant Pathology and Microbiology and Institute for Integrative Genome BiologyUniversity of CaliforniaRiverside, CA92521 USA
Gaba, V., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Gal-On, A., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Facilitators :
From page:
300
To page:
312
(
Total pages:
13
)
Abstract:
RNA-dependent RNA polymerase 1 (RDR1) plays a crucial role in plant defence against viruses. In this study, it was observed that cucumber, Cucumis sativus, uniquely encodes a small gene family of four RDR1 genes. The cucumber RDR1 genes (CsRDR1a, CsRDR1b and duplicated CsRDR1c1/c2) shared 55%-60% homology in their encoded amino acid sequences. In healthy cucumber plants, RDR1a and RDR1b transcripts were expressed at higher levels than transcripts of RDR1c1/c2, which were barely detectable. The expression of all four CsRDR1 genes was induced by virus infection, after which the expression level of CsRDR1b increased 10-20-fold in several virus-resistant cucumber cultivars and in a broad virus-resistant transgenic cucumber line expressing a high level of transgene small RNAs, all without alteration in salicylic acid (SA) levels. By comparison, CsRDR1c1/c2 genes were highly induced (25-1300-fold) in susceptible cucumber cultivars infected with RNA or DNA viruses. Inhibition of RDR1c1/c2 expression led to increased virus accumulation. Ectopic application of SA induced the expression of cucumber RDR1a, RDR1b and RDRc1/c2 genes. A constitutive high level of RDR1b gene expression independent of SA was found to be associated with broad virus resistance. These findings show that multiple RDR1 genes are involved in virus resistance in cucumber and are regulated in a coordinated fashion with different expression profiles. © 2016 BSPP AND JOHN WILEY & SONS LTD.
Note:
Related Files :
Cucumber
gene expression
RDR1
Silencing
virus resistance
Show More
Related Content
More details
DOI :
10.1111/mpp.12518
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29382
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:46
You may also be interested in
Scientific Publication
Differential expression of cucumber RNA-dependent RNA polymerase 1 genes during antiviral defence and resistance
19
Leibman, D., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Kravchik, M., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Wolf, D., Department of Vegetable and Field CropsAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Haviv, S., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Weissberg, M., Department of Fruit Tree SciencesAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Ophir, R., Department of Fruit Tree SciencesAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Paris, H.S., Department of Vegetable Crops and Plant GeneticsAgricultural Research Organization, Newe Ya'ar Research CenterPO Box 1021Ramat 30-095Yishay Israel
Palukaitis, P., Department of Horticultural SciencesSeoul Women's UniversityNowon-gu 01797Seoul South Korea
Ding, S.-W., Department of Plant Pathology and Microbiology and Institute for Integrative Genome BiologyUniversity of CaliforniaRiverside, CA92521 USA
Gaba, V., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Gal-On, A., Department of Plant Pathology and Weed ResearchAgricultural Research Organization, Volcani CenterBet Dagan50250 Israel
Differential expression of cucumber RNA-dependent RNA polymerase 1 genes during antiviral defence and resistance
RNA-dependent RNA polymerase 1 (RDR1) plays a crucial role in plant defence against viruses. In this study, it was observed that cucumber, Cucumis sativus, uniquely encodes a small gene family of four RDR1 genes. The cucumber RDR1 genes (CsRDR1a, CsRDR1b and duplicated CsRDR1c1/c2) shared 55%-60% homology in their encoded amino acid sequences. In healthy cucumber plants, RDR1a and RDR1b transcripts were expressed at higher levels than transcripts of RDR1c1/c2, which were barely detectable. The expression of all four CsRDR1 genes was induced by virus infection, after which the expression level of CsRDR1b increased 10-20-fold in several virus-resistant cucumber cultivars and in a broad virus-resistant transgenic cucumber line expressing a high level of transgene small RNAs, all without alteration in salicylic acid (SA) levels. By comparison, CsRDR1c1/c2 genes were highly induced (25-1300-fold) in susceptible cucumber cultivars infected with RNA or DNA viruses. Inhibition of RDR1c1/c2 expression led to increased virus accumulation. Ectopic application of SA induced the expression of cucumber RDR1a, RDR1b and RDRc1/c2 genes. A constitutive high level of RDR1b gene expression independent of SA was found to be associated with broad virus resistance. These findings show that multiple RDR1 genes are involved in virus resistance in cucumber and are regulated in a coordinated fashion with different expression profiles. © 2016 BSPP AND JOHN WILEY & SONS LTD.
Scientific Publication
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