אסיף מאגר המחקר החקלאי

Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. Grand Nain

Year:

2011

Source of publication :

Authors :

Volume :

20

Co-Authors:

Vishnevetsky, J., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
White Jr., T.L., Tropical Research and Education Center, University of Florida, IFAS, Homestead, FL 33031, United States
Palmateer, A.J., Tropical Research and Education Center, University of Florida, IFAS, Homestead, FL 33031, United States
Flaishman, M., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Cohen, Y., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Elad, Y., Department of Plant Pathology and Weed Research, Institute of Plant Protection, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Velcheva, M., Alberta Research Council, P.O. Bag 4000, T9C 1T4 Vegreville, AB, Canada
Hanania, U., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Sahar, N., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Dgani, O., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel
Perl, A., Department of Fruit Tree Sciences, Institute of Plant Sciences, The Agricultural Research Organization, P.O. Box 6, 50250 Bet-Dagan, Israel

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Abstract:

The most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly Sigatoka Leaf spot disease caused by Mycosphaerella fijiensis. In this study, we developed a transformation system for banana and expressed the endochitinase gene ThEn-42 from Trichoderma harzianum together with the grape stilbene synthase (StSy) gene in transgenic banana plants under the control of the 35S promoter and the inducible PR-10 promoter, respectively. The superoxide dismutase gene Cu,Zn-SOD from tomato, under control of the ubiquitin promoter, was added to this cassette to improve scavenging of free radicals generated during fungal attack. A 4-year field trial demonstrated several transgenic banana lines with improved tolerance to Sigatoka. As the genes conferring Sigatoka tolerance may have a wide range of anti-fungal activities we also inoculated the regenerated banana plants with Botrytis cinerea. The best transgenic lines exhibiting Sigatoka tolerance were also found to have tolerance to B. cinerea in laboratory assays. © 2010 Springer Science+Business Media B.V.

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