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Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species
Year:
1982
Source of publication :
Theoretical and Applied Genetics
Authors :
Spiegel-Roy, Pinchas
;
.
Vardi, Aliza
;
.
Volume :
62
Co-Authors:
Vardi, A., Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Spiegel-Roy, P., Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Galun, E., The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
171
To page:
176
(
Total pages:
6
)
Abstract:
Nucellar callus lines were established from two orange cultivars ('Nucellar Shamouti', 'Shamouti Landau'), three mandarin cultivars ('Murcott', 'Dancy', 'Ponkan') one grapefruit cultivar ('Duncan') and sour orange (Citrus aurantium). These callus lines were initiated from in vitro cultured ovules of young fruits and maintained an embryogenic capacity. The plating efficiencies of protoplasts derived from these calli, as well as those of protoplasts from lemon (cv. 'Villafranca') nucellar callus were differentially affected by the maceration enzymes and by the sugars used as osmotic stabilizers. Plants with normal morphological features were regenerated from cultured protoplasts derived from each of the nucellar callus lines. The establishment of eight new protoplast systems in Citrus paves the way for cell genetics studies and for novel breeding approaches in these economically important orchard trees. © 1982 Springer-Verlag.
Note:
Related Files :
Citrus
Maceration enzymes-Osmotic stabilizers-Plating efficiency
nucellar callus
Plant regeneration
Show More
Related Content
More details
DOI :
10.1007/BF00293354
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29458
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
Scientific Publication
Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species
62
Vardi, A., Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Spiegel-Roy, P., Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Galun, E., The Weizmann Institute of Science, Rehovot, Israel
Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species
Nucellar callus lines were established from two orange cultivars ('Nucellar Shamouti', 'Shamouti Landau'), three mandarin cultivars ('Murcott', 'Dancy', 'Ponkan') one grapefruit cultivar ('Duncan') and sour orange (Citrus aurantium). These callus lines were initiated from in vitro cultured ovules of young fruits and maintained an embryogenic capacity. The plating efficiencies of protoplasts derived from these calli, as well as those of protoplasts from lemon (cv. 'Villafranca') nucellar callus were differentially affected by the maceration enzymes and by the sugars used as osmotic stabilizers. Plants with normal morphological features were regenerated from cultured protoplasts derived from each of the nucellar callus lines. The establishment of eight new protoplast systems in Citrus paves the way for cell genetics studies and for novel breeding approaches in these economically important orchard trees. © 1982 Springer-Verlag.
Scientific Publication
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