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Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.]
Year:
1997
Source of publication :
Current Genetics
Authors :
Freeman, Stanley
;
.
Volume :
32
Co-Authors:
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Redman, R.S., Northwest Biological Science Center, Biological Resources Division, US Geological Survey, 6505 NE 65th, Seattle, WA 98115, United States
Grantham, G., Northwest Biological Science Center, Biological Resources Division, US Geological Survey, 6505 NE 65th, Seattle, WA 98115, United States
Rodriguez, R.J., Department of Plant Pathology, University of California, Riverside, CA 92521, United States
Facilitators :
From page:
152
To page:
156
(
Total pages:
5
)
Abstract:
A 7.4-kilobase (kb) DNA plasmid was isolated from Glomerella musae isolate 927 and designated pGML1. Exonuclease treatments indicated that pGML1 was a linear plasmid with blocked 5' termini. Cell-fractionation experiments combined with sequence-specific PCR amplification revealed that pGML1 resided in mitochondria. The pGML1 plasmid hybridized to cesium chloride-fractionated nuclear DNA but not to A + T-rich mitochondrial DNA. An internal 7.0-kb section of pGML1 was cloned and did not hybridize with either nuclear or mitochondrial DNA from G. musae. Sequence analysis revealed identical terminal inverted repeats (TIR) of 520 bp at the ends of the cloned 7.0-kb section of pGML1. The occurrence of pGML1 did not correspond with the pathogenicity of G. musae on banana fruit. Four additional isolates of G. musae possessed extrachromosomal DNA fragments similar in size and sequence to pGML1.
Note:
Related Files :
Ascomycota
mitochondria
mitochondrial DNA
plant
Plants
Plasmid
Polymerase Chain Reaction
Sequence Analysis, DNA
Show More
Related Content
More details
DOI :
10.1007/s002940050260
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29461
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
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Scientific Publication
Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.]
32
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50250, Israel
Redman, R.S., Northwest Biological Science Center, Biological Resources Division, US Geological Survey, 6505 NE 65th, Seattle, WA 98115, United States
Grantham, G., Northwest Biological Science Center, Biological Resources Division, US Geological Survey, 6505 NE 65th, Seattle, WA 98115, United States
Rodriguez, R.J., Department of Plant Pathology, University of California, Riverside, CA 92521, United States
Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.]
A 7.4-kilobase (kb) DNA plasmid was isolated from Glomerella musae isolate 927 and designated pGML1. Exonuclease treatments indicated that pGML1 was a linear plasmid with blocked 5' termini. Cell-fractionation experiments combined with sequence-specific PCR amplification revealed that pGML1 resided in mitochondria. The pGML1 plasmid hybridized to cesium chloride-fractionated nuclear DNA but not to A + T-rich mitochondrial DNA. An internal 7.0-kb section of pGML1 was cloned and did not hybridize with either nuclear or mitochondrial DNA from G. musae. Sequence analysis revealed identical terminal inverted repeats (TIR) of 520 bp at the ends of the cloned 7.0-kb section of pGML1. The occurrence of pGML1 did not correspond with the pathogenicity of G. musae on banana fruit. Four additional isolates of G. musae possessed extrachromosomal DNA fragments similar in size and sequence to pGML1.
Scientific Publication
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