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A quantitative method for determining soil populations of Streptomyces and differentiating potential potato scab-inducing strains
Year:
1998
Source of publication :
Plant Disease
Authors :
Kritzman, Giora
;
.
Volume :
82
Co-Authors:
Conn, K.L., Agriculture and Agri-Food Canada, Pest Management Research Center, 1391 Sandford St., London, Ont. N5V 4T3, Canada
Leci, E., Agriculture and Agri-Food Canada, Pest Management Research Center, 1391 Sandford St., London, Ont. N5V 4T3, Canada
Kritzman, G., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50 250, Israel
Lazarovits, G., AAFC, PMRC, 1391 Sandford St., London, Ont. N5V 4T3, Canada
Facilitators :
From page:
631
To page:
638
(
Total pages:
8
)
Abstract:
A procedure is described for estimating Streptomyces populations in soil. Soils are air-dried, 10-g quantities are shaken in plastic bags containing 0.1% water agar and homogenized with a Stomacher homogenizer, serial dilutions are plated on a semi-selective culture (STR) medium and incubated for 2 weeks at 22°C, and the Streptomyces colonies are enumerated. Use of STR medium reduced the bacterial and fungal colonies recovered from soil to levels below that of the Streptomyces spp. while not affecting the number of Streptomyces colonies compared with those enumerated on yeast malt extract medium. A procedure for screening large numbers of Streptomyces strains for thaxtomin production, a phytotoxin recognized as a virulence marker in S. scabies, is also described. Strains are grown on oatmeal medium, and the thaxtomin is extracted from the medium by facilitated diffusion and detected by miniature thin layer chromatography. S. scabies and S. acidiscabies strains (approximately 130 from Ontario and 70 from other locations in North America) that produced thaxtomin did not form aerial mycelia or sporulate on STR medium within 2 weeks at 22°C. Ontario S. scabies strains that produced thaxtomin A also produced melanin on STR medium. All S. scabies strains from scab lesions that produced thaxtomin A had this colony morphology, whereas only 4 to 9% of strains from soil with this colony morphology produced thaxtomin A. Using these procedures, we determined that the population of thaxtomin-producing S. scabies in soil from a potato field in Ontario with a history of potato scab was about 20,000 CFU/g soil.
Note:
Related Files :
Canada
Differentiation
fungus detection
oat
plastic
secondary metabolite
soil pollution
Streptomyces acidiscabies
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Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29483
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
Scientific Publication
A quantitative method for determining soil populations of Streptomyces and differentiating potential potato scab-inducing strains
82
Conn, K.L., Agriculture and Agri-Food Canada, Pest Management Research Center, 1391 Sandford St., London, Ont. N5V 4T3, Canada
Leci, E., Agriculture and Agri-Food Canada, Pest Management Research Center, 1391 Sandford St., London, Ont. N5V 4T3, Canada
Kritzman, G., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan 50 250, Israel
Lazarovits, G., AAFC, PMRC, 1391 Sandford St., London, Ont. N5V 4T3, Canada
A quantitative method for determining soil populations of Streptomyces and differentiating potential potato scab-inducing strains
A procedure is described for estimating Streptomyces populations in soil. Soils are air-dried, 10-g quantities are shaken in plastic bags containing 0.1% water agar and homogenized with a Stomacher homogenizer, serial dilutions are plated on a semi-selective culture (STR) medium and incubated for 2 weeks at 22°C, and the Streptomyces colonies are enumerated. Use of STR medium reduced the bacterial and fungal colonies recovered from soil to levels below that of the Streptomyces spp. while not affecting the number of Streptomyces colonies compared with those enumerated on yeast malt extract medium. A procedure for screening large numbers of Streptomyces strains for thaxtomin production, a phytotoxin recognized as a virulence marker in S. scabies, is also described. Strains are grown on oatmeal medium, and the thaxtomin is extracted from the medium by facilitated diffusion and detected by miniature thin layer chromatography. S. scabies and S. acidiscabies strains (approximately 130 from Ontario and 70 from other locations in North America) that produced thaxtomin did not form aerial mycelia or sporulate on STR medium within 2 weeks at 22°C. Ontario S. scabies strains that produced thaxtomin A also produced melanin on STR medium. All S. scabies strains from scab lesions that produced thaxtomin A had this colony morphology, whereas only 4 to 9% of strains from soil with this colony morphology produced thaxtomin A. Using these procedures, we determined that the population of thaxtomin-producing S. scabies in soil from a potato field in Ontario with a history of potato scab was about 20,000 CFU/g soil.
Scientific Publication
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