Abstract:
In studies to determine lipid peroxidation by the accumulation of thiobarbituric acid reactive compounds and conjugated dienes, muscle lipid peroxidation was dependent on the presence of oxygen and free metal ions. Lipid peroxidation in raw and heated samples was prevented by vacuum and ethylenediaminetetraacetic acid (EDTA). In membrane model systems, a low concentration of EDTA inhibited enzymatic and nonenzymatic free metal ion catalysis, and only a very high concentration of EDTA inhibited activated metmyoglobin catalysis. Lipid peroxidation in minced turkey muscle is affected especially by “free” metal ions. © 1988, American Chemical Society. All rights reserved.