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The SV40 DNA template for transcription of late mRNA in viral nucleoprotein complexes
Year:
1977
Source of publication :
Cell
Authors :
Shani, Moshe
;
.
Volume :
11
Co-Authors:
Birkenmeier, E.H., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Radonovich, M.F., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Shani, M., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Salzman, N.P., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Facilitators :
From page:
495
To page:
504
(
Total pages:
10
)
Abstract:
It has been proposed that different patterns of transcription of simian virus 40 (SV40) at early and late stages of infection may be regulated by the utilization of templates with different configurations. Nucleoprotein complexes isolated from SV40-Infected cell nuclei provide a method of characterizing the templates for late viral mRNA synthesis. These complexes contain RNA polymerase activity and synthesize viral RNA in an in vitro reaction. Most of the reaction products remain bound to the DNA templates, and they have been isolated by sedimentation in sucrose gradients both in the presence and in the absence of sodium dodecylsulfate. After treatment of the reaction products with pancreatic ribonuclease A, the fraction of the nascent SV40 RNA containing the 3′ end forms a stable structure with the template DNA and sediments slightly faster than SV40 DNA I in sucrose gradients. These RNA-DNA hybrid molecules are derived from transcriptional intermediates and contain RNAs ranging in size from 35-200 nucleotides. The radioactive label incorporated in the RNA serves as a marker for the DNA template to which it is bound. Sedimentation of the hybrid molecules in sucrose gradients containing ethidium bromide indicates that the small RNA fragment is bound to a DNA template which is a double-stranded, covalently closed circular molecule with negative superhelical turns. The data demonstrate that SV40 DNA I serves as a template for transcription during the late phase of lytic infection. © 1977.
Note:
Related Files :
DNA, Circular
DNA-Directed RNA Polymerases
microorganism
Nucleoproteins
virus DNA
Show More
Related Content
More details
DOI :
10.1016/0092-8674(77)90067-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29546
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:47
Scientific Publication
The SV40 DNA template for transcription of late mRNA in viral nucleoprotein complexes
11
Birkenmeier, E.H., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Radonovich, M.F., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Shani, M., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
Salzman, N.P., Laboratory of Biology, Viruses National Institute of Allergy and Infectious Diseases Bethesda, MD 20014, United States
The SV40 DNA template for transcription of late mRNA in viral nucleoprotein complexes
It has been proposed that different patterns of transcription of simian virus 40 (SV40) at early and late stages of infection may be regulated by the utilization of templates with different configurations. Nucleoprotein complexes isolated from SV40-Infected cell nuclei provide a method of characterizing the templates for late viral mRNA synthesis. These complexes contain RNA polymerase activity and synthesize viral RNA in an in vitro reaction. Most of the reaction products remain bound to the DNA templates, and they have been isolated by sedimentation in sucrose gradients both in the presence and in the absence of sodium dodecylsulfate. After treatment of the reaction products with pancreatic ribonuclease A, the fraction of the nascent SV40 RNA containing the 3′ end forms a stable structure with the template DNA and sediments slightly faster than SV40 DNA I in sucrose gradients. These RNA-DNA hybrid molecules are derived from transcriptional intermediates and contain RNAs ranging in size from 35-200 nucleotides. The radioactive label incorporated in the RNA serves as a marker for the DNA template to which it is bound. Sedimentation of the hybrid molecules in sucrose gradients containing ethidium bromide indicates that the small RNA fragment is bound to a DNA template which is a double-stranded, covalently closed circular molecule with negative superhelical turns. The data demonstrate that SV40 DNA I serves as a template for transcription during the late phase of lytic infection. © 1977.
Scientific Publication
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