אסיף מאגר המחקר החקלאי

A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes

Year:

2010

Source of publication :

Authors :

Volume :

121

Co-Authors:

Harel-Beja, R., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Tzuri, G., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Portnoy, V., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Lotan-Pompan, M., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Lev, S., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Cohen, S., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Dai, N., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Yeselson, L., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Meir, A., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Libhaber, S.E., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Avisar, E., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Melame, T., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
van Koert, P., Keygene N.V., Business Park, 6708 Wageningen, Netherlands
Verbakel, H., Keygene N.V., Business Park, 6708 Wageningen, Netherlands
Hofstede, R., Keygene N.V., Business Park, 6708 Wageningen, Netherlands
Volpin, H., Keygene N.V., Business Park, 6708 Wageningen, Netherlands
Oliver, M., Syngenta Seeds S.A.S., 31790 Saint Sauveur, France
Fougedoire, A., HM Clause, Davis, CA 95618, United States
Stalh, C., HM Clause, Davis, CA 95618, United States
Fauve, J., Clause, 13210 St Rémy de Provence, France
Copes, B., HM Clause, Davis, CA 95618, United States
Fei, Z., United States Department of Agriculture, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, United States
Giovannoni, J., United States Department of Agriculture, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, United States
Ori, N., The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100 Rehovot, Israel
Lewinsohn, E., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Sherman, A., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Burger, J., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Tadmor, Y., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Schaffer, A.A., Department of Vegetable Research, Agricultural Research Organization, Volcani Research Center, 50250 Bet Dagan, Israel
Katzir, N., Department of Vegetable Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel

Facilitators :

Link :

Abstract:

A genetic map of melon enriched for fruit traits was constructed, using a recombinant inbred (RI) population developed from a cross between representatives of the two subspecies of Cucumis melo L.: PI 414723 (subspecies agrestis) and 'Dulce' (subspecies melo). Phenotyping of 99 RI lines was conducted over three seasons in two locations in Israel and the US. The map includes 668 DNA markers (386 SSRs, 76 SNPs, six INDELs and 200 AFLPs), of which 160 were newly developed from fruit ESTs. These ESTs include candidate genes encoding for enzymes of sugar and carotenoid metabolic pathways that were cloned from melon cDNA or identified through mining of the International Cucurbit Genomics Initiative database (http://www. icugi. org/). The map covers 1,222 cM with an average of 2.672 cM between markers. In addition, a skeleton physical map was initiated and 29 melon BACs harboring fruit ESTs were localized to the 12 linkage groups of the map. Altogether, 44 fruit QTLs were identified: 25 confirming QTLs described using other populations and 19 newly described QTLs. The map includes QTLs for fruit sugar content, particularly sucrose, the major sugar affecting sweetness in melon fruit. Six QTLs interacting in an additive manner account for nearly all the difference in sugar content between the two genotypes. Three QTLs for fruit flesh color and carotenoid content were identified. Interestingly, no clear colocalization of QTLs for either sugar or carotenoid content was observed with over 40 genes encoding for enzymes involved in their metabolism. The RI population described here provides a useful resource for further genomics and metabolomics studies in melon, as well as useful markers for breeding for fruit quality. © 2010 Springer-Verlag.

Note:

Related Files :

Related Content

More details

You may also be interested in