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Dormancy in grape buds: Isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release
Year:
2002
Source of publication :
Plant Science
Authors :
Eyal, Yoram
;
.
Ogrodovitch, Aliza
;
.
Or, Etti
;
.
Vilozny, Iris
;
.
Volume :
162
Co-Authors:
Or, E., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Vilozny, I., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Fennell, A., Horticulture, Forestry, Landscape, and Parks Department, South Dakota State University, Box 2140A, NPB 201, Brookings, SD 57007, United States
Eyal, Y., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Ogrodovitch, A., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Facilitators :
From page:
121
To page:
130
(
Total pages:
10
)
Abstract:
The mechanism by which hydrogen cyanamide (HC) exerts its dormancy-breaking effect is not clear, but it has been shown to inactivate catalase in grape buds shortly after its application. Recently, we showed that some potential components in the process leading to dormancy release are induced at the level of gene expression following application of HC. Therefore, we inquired whether changes in catalase activity following HC application are also accompanied by changes in the expression of this gene. We report here the isolation of the first cDNA clone encoding a grape catalase and the characterization of its expression following artificial and natural induction of dormancy release. The sharp decline in the level of catalase transcript, following induction of dormancy release by HC, correlates with earlier findings showing a major decrease in catalase activity in the buds following HC application. This may suggest that HC exert its effect on catalase activity, at least partially, through regulation of catalase gene expression. On the other hand, the expression pattern of catalase in buds throughout the natural dormancy cycle was of a constitutive nature and did not correlate with earlier findings showing decreased activity towards dormancy release. The discrepancy between the data from artificial and natural induction systems is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Note:
Related Files :
cloning
cyanamide
DNA
gene expression
Genes
grape
Induction systems
plant growth
Vitis
Show More
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More details
DOI :
10.1016/S0168-9452(01)00542-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29629
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:48
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Scientific Publication
Dormancy in grape buds: Isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release
162
Or, E., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Vilozny, I., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Fennell, A., Horticulture, Forestry, Landscape, and Parks Department, South Dakota State University, Box 2140A, NPB 201, Brookings, SD 57007, United States
Eyal, Y., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Ogrodovitch, A., Department of Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Dormancy in grape buds: Isolation and characterization of catalase cDNA and analysis of its expression following chemical induction of bud dormancy release
The mechanism by which hydrogen cyanamide (HC) exerts its dormancy-breaking effect is not clear, but it has been shown to inactivate catalase in grape buds shortly after its application. Recently, we showed that some potential components in the process leading to dormancy release are induced at the level of gene expression following application of HC. Therefore, we inquired whether changes in catalase activity following HC application are also accompanied by changes in the expression of this gene. We report here the isolation of the first cDNA clone encoding a grape catalase and the characterization of its expression following artificial and natural induction of dormancy release. The sharp decline in the level of catalase transcript, following induction of dormancy release by HC, correlates with earlier findings showing a major decrease in catalase activity in the buds following HC application. This may suggest that HC exert its effect on catalase activity, at least partially, through regulation of catalase gene expression. On the other hand, the expression pattern of catalase in buds throughout the natural dormancy cycle was of a constitutive nature and did not correlate with earlier findings showing decreased activity towards dormancy release. The discrepancy between the data from artificial and natural induction systems is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
Scientific Publication
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