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Effect of flagella expression on adhesion of Achromobacter piechaudii to chalk surfaces
Year:
2008
Source of publication :
Journal of Applied Microbiology
Authors :
Saadi, Ibrahim
;
.
Volume :
105
Co-Authors:
Aims: To examine flagella role and cell motility in adhesion of Achromobacter piechaudii to chalk. Methods and Results: Transmission electron microscopy revealed that stationary cells have thicker and longer flagella than logarithmic cells. SDS-PAGE analysis showed that flagellin was more abundant in stationary cells than logarithmic ones. Sonication or inhibition of flagellin synthesis caused a 30% reduction in adhesion to chalk. Preincubation of chalk with flagella extracts reduced adhesion, by 50%. Three motility mutants were isolated. Mutants 94 and 153 were nonmotile, expressed normal levels of flagellin, have regular flagella and exhibited reduced adhesion. Mutant 208 expressed low levels of flagellin, no flagella and a spherical cell shape but with normal adhesion capacity. Conclusions: Multiple cell surface factors affect the adhesion efficiency to chalk. Flagella per se through physical interaction and through cell motility contribute to the adhesion process. The adhesion behaviour of mutant 208 suggests that cell shape can compensate for flagellar removal and motility. Significance and Impact of the Study: Physiological status affects bacterial cell surface properties and hence adhesion efficiency to chalk. This interaction is essential to sustain biodegradation activities and thus, remediation of contaminated chalk aquifers. © 2008 The Authors.
Facilitators :
From page:
2009
To page:
2014
(
Total pages:
6
)
Abstract:
Adhesion flagellum; Gray chalk; Groundwater; Industrial wastewater; Tribromophenol degradation; White chalk
Note:
Related Files :
bacteria
Biodegradation
Microbial degradation
mutation
phenol
Ultraviolet Rays
Wastewater
Show More
Related Content
More details
DOI :
https://www.scopus.com/inward/record.uri?eid=2-s2.0-56649124733&doi=10.1111%2fj.1365-2672.2008.03930.x&partnerID=40&md5=cb69b0c94eae6adb90d59a3578cf420c
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29653
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:48
Scientific Publication
Effect of flagella expression on adhesion of Achromobacter piechaudii to chalk surfaces
105
Aims: To examine flagella role and cell motility in adhesion of Achromobacter piechaudii to chalk. Methods and Results: Transmission electron microscopy revealed that stationary cells have thicker and longer flagella than logarithmic cells. SDS-PAGE analysis showed that flagellin was more abundant in stationary cells than logarithmic ones. Sonication or inhibition of flagellin synthesis caused a 30% reduction in adhesion to chalk. Preincubation of chalk with flagella extracts reduced adhesion, by 50%. Three motility mutants were isolated. Mutants 94 and 153 were nonmotile, expressed normal levels of flagellin, have regular flagella and exhibited reduced adhesion. Mutant 208 expressed low levels of flagellin, no flagella and a spherical cell shape but with normal adhesion capacity. Conclusions: Multiple cell surface factors affect the adhesion efficiency to chalk. Flagella per se through physical interaction and through cell motility contribute to the adhesion process. The adhesion behaviour of mutant 208 suggests that cell shape can compensate for flagellar removal and motility. Significance and Impact of the Study: Physiological status affects bacterial cell surface properties and hence adhesion efficiency to chalk. This interaction is essential to sustain biodegradation activities and thus, remediation of contaminated chalk aquifers. © 2008 The Authors.
Effect of flagella expression on adhesion of Achromobacter piechaudii to chalk surfaces
Adhesion flagellum; Gray chalk; Groundwater; Industrial wastewater; Tribromophenol degradation; White chalk
Scientific Publication
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